3-dimensional imaging at nanometer resolutions

Werner, James H; Goodwin, Peter M; Shreve, Andrew P

3-dimensional imaging at nanometer resolutions

Patent · 08 March 2010

OSTI ID:1014498

Werner, James H ^[1]; Goodwin, Peter M ^[1]; Shreve, Andrew P ^[2]

Los Alamos, NM
Santa Fe, NM

An apparatus and method for enabling precise, 3-dimensional, photoactivation localization microscopy (PALM) using selective, two-photon activation of fluorophores in a single z-slice of a sample in cooperation with time-gated imaging for reducing the background radiation from other image planes to levels suitable for single-molecule detection and spatial location, are described.

Research Organization:: Los Alamos National Security, LLC (Los Alamos, NM)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC52-06NA25396

Assignee:: Los Alamos National Security, LLC (Los Alamos, NM)

Patent Number(s):: 7,675,045

Application Number:: U S Patent Application 12/248,834

OSTI ID:: 1014498

Country of Publication:: United States

Language:: English

References (8)

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3-dimensional imaging at nanometer resolutions

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