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Isolated polypeptide having arabinofuranosidase activity

Patent ·
OSTI ID:1014180
Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry. TABLE-US-00001 cip1 cDNA sequence (SEQ ID NO: 1) GACTAGTTCA TAATACAGTA GTTGAGTTCA TAGCAACTTC 50 ACTCTCTAGC TGAACAAATT ATCTGCGCAA ACATGGTTCG CCGGACTGCT 100 CTGCTGGCCC TTGGGGCTCT CTCAACGCTC TCTATGGCCC AAATCTCAGA 150 CGACTTCGAG TCGGGCTGGG ATCAGACTAA ATGGCCCATT TCGGCACCAG 200 ACTGTAACCA GGGCGGCACC GTCAGCCTCG ACACCACAGT AGCCCACAGC 250 GGCAGCAACT CCATGAAGGT CGTTGGTGGC CCCAATGGCT ACTGTGGACA 300 CATCTTCTTC GGCACTACCC AGGTGCCAAC TGGGGATGTA TATGTCAGAG 350 CTTGGATTCG GCTTCAGACT GCTCTCGGCA GCAACCACGT CACATTCATC 400 ATCATGCCAG ACACCGCTCA GGGAGGGAAG CACCTCCGAA TTGGTGGCCA 450 AAGCCAAGTT CTCGACTACA ACCGCGAGTC CGACGATGCC ACTCTTCCGG 500 ACCTGTCTCC CAACGGCATT GCCTCCACCG TCACTCTGCC TACCGGCGCG 550 TTCCAGTGCT TCGAGTACCA CCTGGGCACT GACGGAACCA TCGAGACGTG 600 GCTCAACGGC AGCCTCATCC CGGGCATGAC CGTGGGCCCT GGCGTCGACA 650 ATCCAAACGA CGCTGGCTGG ACGAGGGCCA GCTATATTCC GGAGATCACC 700 GGTGTCAACT TTGGCTGGGA GGCCTACAGC GGAGACGTCA ACACCGTCTG 750 GTTCGACGAC ATCTCGATTG CGTCGACCCG CGTGGGATGC GGCCCCGGCA 800 GCCCCGGCGG TCCTGGAAGC TCGACGACTG GGCGTAGCAG CACCTCGGGC 850 CCGACGAGCA CTTCGAGGCC AAGCACCACC ATTCCGCCAC CGACTTCCAG 900 GACAACGACC GCCACGGGTC CGACTCAGAC ACACTATGGC CAGTGCGGAG 1000 GGATTGGTTA CAGCGGGCCT ACGGTCTGCG CGAGCGGCAC GACCTGCCAG 1050 GTCCTGAACC CATACTACTC CCAGTGCTTA TAAGGGGATG AGCATGGAGT 1100 GAAGTGAAGT GAAGTGGAGA GAGTTGAAGT GGCATTGCGC TCGGCTGGGT 1150 AGATAAAAGT CAGCAGCTAT GAATACTCTA TGTGATGCTC ATTGGCGTGT 1200 ACGTTTTAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 1250 AAAAAAAAAA AAAAAAAAAG GGGGCGGCCG C 1271
Research Organization:
Danisco US Inc. (Palo Alto, CA)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-99GO10337;
Assignee:
Danisco US Inc. (Palo Alto, CA)
Patent Number(s):
7,666,648
Application Number:
US Patent Application 10/555,358
OSTI ID:
1014180
Country of Publication:
United States
Language:
English

References (34)

A complete protein pattern of cellulase and hemicellulase genes in the filamentous fungusTrichoderma reesei journal November 2006
Gene Transfer and Expression book January 1990
Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins journal August 1993
Deacetylation of xylans by acetyl esterases of Trichoderma reesei journal August 1990
Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations journal July 1984
Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation journal January 1991
Characterization of cellulases by HPLC separation journal January 1983
Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase journal May 2002
Developments in Directed Evolution for Improving Enzyme Functions journal August 2007
Separation of endo- and exo-type cellulases using a new affinity chromatography method journal April 1984
Acetyl xylan esterases in fungal cellulolytic systems journal July 1985
[57] Rethinking your purification procedure book January 1990
Characteristics of fungal cellulases journal January 1991
Acetyl xylan esterase II from Penicillium purpurogenum is similar to an esterase from Trichoderma reesei but lacks a cellulose binding domain journal February 1998
Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography journal January 1987
Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography journal May 1998
Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction journal December 1999
[79] Purification of all glycolytic enzymes from one muscle extract journal January 1982
α-l-Arabinofuranosidases journal August 2000
Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast journal April 2001
Structures and mechanisms of glycosyl hydrolases journal September 1995
Identification of genes encoding microbial glucuronoyl esterases journal July 2007
Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning journal November 1999
Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27 journal October 1983
A classification of glycosyl hydrolases based on amino acid sequence similarities journal December 1991
New families in the classification of glycosyl hydrolases based on amino acid sequence similarities journal August 1993
Transcriptional Regulation of Biomass-degrading Enzymes in the Filamentous Fungus Trichoderma reesei journal June 2003
Structure of Cellulases and Their Applications journal April 1997
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs journal September 1997
Acetyl Xylan Esterase from Trichoderma reesei Contains an Active-Site Serine Residue and a Cellulose-Binding Domain journal May 1996
Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei journal September 2000
Cloning of genes encoding alpha-L-arabinofuranosidase and beta-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae. journal January 1996
An α-l-Arabinofuranosidase fromTrichoderma reesei Containing a Noncatalytic Xylan-Binding Domain journal September 1999
Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. journal November 1991

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