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Materials and methods for efficient lactic acid production

Patent ·
OSTI ID:1013854
 [1];  [2];  [2];  [2];  [2];  [2]
  1. Sycamore, IL
  2. Gainesville, FL
+ Show Author Affiliations

The present invention provides derivatives of ethanologenic Escherichia coli K011 constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.

Research Organization:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-96ER20222
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Patent Number(s):
7,629,162
Application Number:
US Patent Application 11/501,137
OSTI ID:
1013854
Country of Publication:
United States
Language:
English

References (8)

Fermentation of 10% (w/v) Sugar to D(−)-Lactate by Engineered Escherichia coli B journal December 2005
Methylglyoxal Bypass Identified as Source of Chiral Contamination in l(+) and d(−)-lactate Fermentations by Recombinant Escherichia coli journal July 2006
Homofermentative Production of d- orl-Lactate in Metabolically Engineered Escherichia coli RR1 journal April 1999
Betaine Tripled the Volumetric Productivity of d(-)-lactate by Escherichia coli Strain SZ132 in Mineral Salts Medium journal May 2006
Production of Optically Pure D-Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110 journal January 2003
Fermentation of 12% (w/v) Glucose to 1.2 m Lactate by Escherichia coli Strain SZ194 using Mineral Salts Medium journal May 2006
From famine to feast: the role of methylglyoxal production in Escherichia coli journal February 1998
Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars journal October 2001

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