[Enhancement of photoassimilate utilization by manipulation of the ADPglucose pyrophosphorylase gene]. Summary of progress, [April 15, 1991--April 14, 1992]

Okita, T W

doi:10.2172/10137758

Title: [Enhancement of photoassimilate utilization by manipulation of the ADPglucose pyrophosphorylase gene]. Summary of progress, [April 15, 1991--April 14, 1992]

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Abstract

The long term aim of this project is to assess the feasibility of increasing the conversion of photosynthate into starch via manipulation of genes encoding enzymes that may be rate-limiting in starch biosynthesis. In developing storage tissues such as tubers, starch biosynthesis is regulated by the gene activation and expression of ADPglucose pyrophosphorylase, starch synthase, branching enzyme and other ancillary starch modifying enzymes, as well as the allosteric-controlled behavior of ADPglucose pyrophosphorylase activity. In view of the regulatory role of ADPglucose pyrophosphorylase in starch biosynthesis at both the genetic and biochemical level, we have focused our attention on the genes that encode for this enzyme in potato tubers. The proposed objectives of the grant were to (1) analyze the structure of the tuber enzyme, (2) isolate and characterize the structure of its genes, and (3) identify the regulatory elements controlling ADPglucose pyrophosphorylase during plant development. During the last two and 1/2 years we have met or have made considerable progress in achieving these objectives as discussed in more detail below.

Authors:: Okita, T W

Publication Date:: Thu Dec 31 00:00:00 EST 1992

Research Org.:: Washington State Univ., Pullman, WA (United States). Inst. of Biological Chemistry

Sponsoring Org.:: USDOE, Washington, DC (United States)

OSTI Identifier:: 10137758

Report Number(s):: DOE/ER/13699-5
ON: DE94009198; TRN: 94:004278

DOE Contract Number:: FG06-87ER13699

Resource Type:: Technical Report

Resource Relation:: Other Information: PBD: [1992]

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; STARCH; BIOSYNTHESIS; POTATOES; GENETIC ENGINEERING; ADP; GENE REGULATION; STRUCTURE-ACTIVITY RELATIONSHIPS; PROGRESS REPORT; ENDOSPERM; ENZYME ACTIVITY; TUBERS; 550400; 550200; GENETICS; BIOCHEMISTRY

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                    Okita, T W. [Enhancement of photoassimilate utilization by manipulation of the ADPglucose pyrophosphorylase gene]. Summary of progress, [April 15, 1991--April 14, 1992].  United States: N. p., 1992. 
        Web.  doi:10.2172/10137758.

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                    Okita, T W. [Enhancement of photoassimilate utilization by manipulation of the ADPglucose pyrophosphorylase gene]. Summary of progress, [April 15, 1991--April 14, 1992].  United States.  https://doi.org/10.2172/10137758

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                    Okita, T W. 1992.  
        "[Enhancement of photoassimilate utilization by manipulation of the ADPglucose pyrophosphorylase gene]. Summary of progress, [April 15, 1991--April 14, 1992]".  United States.  https://doi.org/10.2172/10137758.  https://www.osti.gov/servlets/purl/10137758.

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@article{osti_10137758,

  title        = {[Enhancement of photoassimilate utilization by manipulation of the ADPglucose pyrophosphorylase gene]. Summary of progress, [April 15, 1991--April 14, 1992]},

  author       = {Okita, T W},

  abstractNote = {The long term aim of this project is to assess the feasibility of increasing the conversion of photosynthate into starch via manipulation of genes encoding enzymes that may be rate-limiting in starch biosynthesis. In developing storage tissues such as tubers, starch biosynthesis is regulated by the gene activation and expression of ADPglucose pyrophosphorylase, starch synthase, branching enzyme and other ancillary starch modifying enzymes, as well as the allosteric-controlled behavior of ADPglucose pyrophosphorylase activity. In view of the regulatory role of ADPglucose pyrophosphorylase in starch biosynthesis at both the genetic and biochemical level, we have focused our attention on the genes that encode for this enzyme in potato tubers. The proposed objectives of the grant were to (1) analyze the structure of the tuber enzyme, (2) isolate and characterize the structure of its genes, and (3) identify the regulatory elements controlling ADPglucose pyrophosphorylase during plant development. During the last two and 1/2 years we have met or have made considerable progress in achieving these objectives as discussed in more detail below.},

  doi          = {10.2172/10137758},

  url          = {https://www.osti.gov/biblio/10137758},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Dec 31 00:00:00 EST 1992},

  month        = {Thu Dec 31 00:00:00 EST 1992}

}

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