Replica amplification of nucleic acid arrays

Patent · 31 August 2010

OSTI ID:1013607

Church, George M ^[1]; Mitra, Robi D ^[2]

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1. Brookline, MA
2. Chestnut Hill, MA

Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.

• Research Organization: President and Fellows of Harvard College (Cambridge, MA)
• Sponsoring Organization: USDOE
• DOE Contract Number: FG02-87ER60565
• Assignee: President and Fellows of Harvard College (Cambridge, MA)
• Patent Number(s): 7,785,790
• Application Number: 11/745,806
• OSTI ID: 1013607
• Country of Publication: United States
• Language: English