Enzyme system comprising an enzyme bonded in a porous matrix

Ackerman, Eric; Liu, Jun

Enzyme system comprising an enzyme bonded in a porous matrix

Patent · 07 December 2010

OSTI ID:1011441

Ackerman, Eric ^[1]; Liu, Jun ^[2]

Richland, WA
West Richland, WA

A protein system is described in which a protein is bound within a matrix material that has pores that are sized to achieve excellent properties such as: activity, protein density, and stability. In a preferred embodiment, the pore sizes range from 50 to 400 .ANG.. One protein that has demonstrated surprisingly good results in this system is OPH. This protein is known to degrade organophosphorus compounds such as are found in chemical weapons and pesticides. Novel methods of forming the protein system and methods of making OPH are also described.

View Patent

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC06-76RL01830

Assignee:: Battelle Memorial Institute (Richland, WA)

Patent Number(s):: 7,846,684

Application Number:: 09/791,138

OSTI ID:: 1011441

Country of Publication:: United States

Language:: English

References (18)

Recent Progress on Immobilization of Enzymes on Molecular Sieves for Reactions in Organic Solvents Yan, Al-Xin; Li, Xuan-Wen; Ye, Yun-Hua Applied Biochemistry and Biotechnology, Vol. 101, Issue 2 https://doi.org/10.1385/ABAB:101:2:113	journal	January 2002
Cytochrome c immobilization into mesoporous molecular sieves Washmon-Kriel, Lisa; Jimenez, Victoria L.; Balkus, Kenneth J. Journal of Molecular Catalysis B: Enzymatic, Vol. 10, Issue 5 https://doi.org/10.1016/S1381-1177(99)00123-X	journal	October 2000
Enzyme immobilisation using SBA-15 mesoporous molecular sieves with functionalised surfaces Yiu, Humphrey H. P.; Wright, Paul A.; Botting, Nigel P. Journal of Molecular Catalysis B: Enzymatic, Vol. 15, Issue 1-3 https://doi.org/10.1016/S1381-1177(01)00011-X	journal	September 2001
Entrapping Enzyme in a Functionalized Nanoporous Support Lei, Chenghong; Shin, Yongsoon; Liu, Jun Journal of the American Chemical Society, Vol. 124, Issue 38, p. 11242-11243 https://doi.org/10.1021/ja026855o	journal	September 2002
Immobilized enzymes in ordered mesoporous silica materials and improvement of their stability and catalytic activity in an organic solvent Takahashi, Haruo; Li, Bo; Sasaki, Toshiya Microporous and Mesoporous Materials, Vol. 44-45 https://doi.org/10.1016/S1387-1811(01)00257-8	journal	April 2001
Enhanced Enzymatic Reactions in a Microchannel Reactor Kanno, Kenichi; Kawazumi, Hirofumi; Miyazaki, Masaya Australian Journal of Chemistry, Vol. 55, Issue 11 https://doi.org/10.1071/CH02171	journal	January 2002
Functionalized Monolayers on Ordered Mesoporous Supports Feng, X.; Fryxell, G. E.; Wang, L. -Q. Science, Vol. 276, Issue 5314 https://doi.org/10.1126/science.276.5314.923	journal	May 1997
Enzyme immobilization in MCM-41 molecular sieve Díaz, J. Felipe; Balkus, Kenneth J. Journal of Molecular Catalysis B: Enzymatic, Vol. 2, Issue 2-3 https://doi.org/10.1016/S1381-1177(96)00017-3	journal	December 1996
Development of sensors for direct detection of organophosphates. Part I: immobilization, characterization and stabilization of acetylcholinesterase and organophosphate hydrolase on silica supports Singh, A. K.; Flounders, A. W.; Volponi, J. V. Biosensors and Bioelectronics, Vol. 14, Issue 8-9 https://doi.org/10.1016/S0956-5663(99)00044-5	journal	December 1999
Enzyme immobilisation using siliceous mesoporous molecular sieves Yiu, Humphrey H. P.; Wright, Paul A.; Botting, Nigel P. Microporous and Mesoporous Materials, Vol. 44-45 https://doi.org/10.1016/S1387-1811(01)00258-X	journal	April 2001
Porous zirconia: a new support material for enzyme immobilization Huckel, Marion; Wirth, Hans-Jürgen; Hearn, Milton T. W. Journal of Biochemical and Biophysical Methods, Vol. 31, Issue 3-4 https://doi.org/10.1016/0165-022X(95)00035-P	journal	February 1996
Biosensor for direct determination of organophosphate nerve agents. 1. Potentiometric enzyme electrode Mulchandani, Priti; Mulchandani, Ashok; Kaneva, Irina Biosensors and Bioelectronics, Vol. 14, Issue 1 https://doi.org/10.1016/S0956-5663(98)00096-7	journal	January 1999
Mesoporous molecular sieve immobilized enzymes Gimon-Kinsel, M. E.; Jimenez, V. L.; Washmon, L. Studies in Surface Science and Catalysis https://doi.org/10.1016/S0167-2991(98)81014-8	book	January 1998
Technology and Applications of Microengineered Reactors Gavriilidis, A.; Angeli, P.; Cao, E. Chemical Engineering Research and Design, Vol. 80, Issue 1 https://doi.org/10.1205/026387602753393196	journal	January 2002
Mesoporous Silicate Sequestration and Release of Proteins Han, Yong-Jin; Stucky, Galen D.; Butler, Alison Journal of the American Chemical Society, Vol. 121, Issue 42 https://doi.org/10.1021/ja992138r	journal	October 1999
Reusable immobilized enzyme/polyurethane sponge for removal and detoxification of localized organophosphate pesticide spills Havens, Patrick L.; Rase, Howard F. Industrial & Engineering Chemistry Research, Vol. 32, Issue 10 https://doi.org/10.1021/ie00022a009	journal	October 1993
Catalytic Activity in Organic Solvents and Stability of Immobilized Enzymes Depend on the Pore Size and Surface Characteristics of Mesoporous Silica Takahashi, Haruo; Li, Bo; Sasaki, Toshiya Chemistry of Materials, Vol. 12, Issue 11 https://doi.org/10.1021/cm000487a	journal	November 2000
Molecular Assembly in Ordered Mesoporosity: A New Class of Highly Functional Nanoscale Materials Liu, Jun; Shin, Yongsoon; Nie, Zimin The Journal of Physical Chemistry A, Vol. 104, Issue 36, p. 8328-8339 https://doi.org/10.1021/jp0009812	journal	September 2000

Similar Records

Entrapping Enzyme in a Functionalized Nanoporous Support

Journal Article · 2002 · Journal of the American Chemical Society · OSTI ID:860119

Gas phase fractionation method using porous ceramic membrane

Patent · 1995 · OSTI ID:870271

Solid polymer electrolyte composite membrane comprising laser micromachined porous support

Patent · 2011 · OSTI ID:1013419

Enzyme system comprising an enzyme bonded in a porous matrix

Citation Formats

References (18)

Similar Records

Related Subjects