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Title: Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source

Abstract

Pharmaceutical research depends on macromolecular crystallography as a tool in drug design and development. To solve the de novo three-dimensional atomic structure of a protein, it is essential to know the phases of the X-rays scattered by a protein crystal. Experimental phases can be obtained from multiwavelength anomalous dispersion (MAD) experiments. Dedicated to macromolecular crystallography, the IMCA-CAT bending magnet beamline at sector 17 of the Advanced Photon Source (APS) was upgraded to provide the energy resolution required to successfully perform synchrotron radiation-based MAD phasing of protein crystal structures. A collimating mirror was inserted into the beam path upstream of a double-crystal monochromator, thus increasing the monochromatic beam throughput in a particular bandwidth without sacrificing the energy resolution of the system. The beam is focused horizontally by a sagittally bent crystal and vertically by a cylindrically bent mirror, delivering a beam at the sample of 130 {micro}m (vertically) x 250 {micro}m (horizontally) FWHM. As a result of the upgrade, the beamline now operates with an energy range of 7.5 x 17.5 keV, delivers 8 x 10{sup +11} photons/sec at 12.398 keV at the sample, and has an energy resolution of {delta}E/E = 1.45 x 10{sup -4} at 10 keV, which ismore » suitable for MAD experiments.« less

Authors:
; ; ; ; ; ; ; ;  [1]
  1. (UC)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1009019
Resource Type:
Conference
Resource Relation:
Conference: Ninth International Conference on Synchrotron Radiation Instrumentation;May 28 - June 2, 2006;Daegu, South Korea
Country of Publication:
United States
Language:
ENGLISH
Subject:
43 PARTICLE ACCELERATORS; 36 MATERIALS SCIENCE; ADVANCED PHOTON SOURCE; BENDING; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DESIGN; DRUGS; ENERGY RANGE; ENERGY RESOLUTION; MAGNETS; MIRRORS; PROTEINS; SYNCHROTRON RADIATION; SYNCHROTRONS

Citation Formats

Koshelev, I., Huang, R., Graber, T., Meron, M., Muir, J.L., Lavender, W., Battaile, K., Mulichak, A.M., and Keefe, L.J. Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source. United States: N. p., 2007. Web. doi:10.1063/1.2436191.
Koshelev, I., Huang, R., Graber, T., Meron, M., Muir, J.L., Lavender, W., Battaile, K., Mulichak, A.M., & Keefe, L.J. Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source. United States. doi:10.1063/1.2436191.
Koshelev, I., Huang, R., Graber, T., Meron, M., Muir, J.L., Lavender, W., Battaile, K., Mulichak, A.M., and Keefe, L.J. Tue . "Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source". United States. doi:10.1063/1.2436191.
@article{osti_1009019,
title = {Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source},
author = {Koshelev, I. and Huang, R. and Graber, T. and Meron, M. and Muir, J.L. and Lavender, W. and Battaile, K. and Mulichak, A.M. and Keefe, L.J.},
abstractNote = {Pharmaceutical research depends on macromolecular crystallography as a tool in drug design and development. To solve the de novo three-dimensional atomic structure of a protein, it is essential to know the phases of the X-rays scattered by a protein crystal. Experimental phases can be obtained from multiwavelength anomalous dispersion (MAD) experiments. Dedicated to macromolecular crystallography, the IMCA-CAT bending magnet beamline at sector 17 of the Advanced Photon Source (APS) was upgraded to provide the energy resolution required to successfully perform synchrotron radiation-based MAD phasing of protein crystal structures. A collimating mirror was inserted into the beam path upstream of a double-crystal monochromator, thus increasing the monochromatic beam throughput in a particular bandwidth without sacrificing the energy resolution of the system. The beam is focused horizontally by a sagittally bent crystal and vertically by a cylindrically bent mirror, delivering a beam at the sample of 130 {micro}m (vertically) x 250 {micro}m (horizontally) FWHM. As a result of the upgrade, the beamline now operates with an energy range of 7.5 x 17.5 keV, delivers 8 x 10{sup +11} photons/sec at 12.398 keV at the sample, and has an energy resolution of {delta}E/E = 1.45 x 10{sup -4} at 10 keV, which is suitable for MAD experiments.},
doi = {10.1063/1.2436191},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 15 00:00:00 EDT 2007},
month = {Tue May 15 00:00:00 EDT 2007}
}

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