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Title: Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source

Abstract

Pharmaceutical research depends on macromolecular crystallography as a tool in drug design and development. To solve the de novo three-dimensional atomic structure of a protein, it is essential to know the phases of the X-rays scattered by a protein crystal. Experimental phases can be obtained from multiwavelength anomalous dispersion (MAD) experiments. Dedicated to macromolecular crystallography, the IMCA-CAT bending magnet beamline at sector 17 of the Advanced Photon Source (APS) was upgraded to provide the energy resolution required to successfully perform synchrotron radiation-based MAD phasing of protein crystal structures. A collimating mirror was inserted into the beam path upstream of a double-crystal monochromator, thus increasing the monochromatic beam throughput in a particular bandwidth without sacrificing the energy resolution of the system. The beam is focused horizontally by a sagittally bent crystal and vertically by a cylindrically bent mirror, delivering a beam at the sample of 130 {micro}m (vertically) x 250 {micro}m (horizontally) FWHM. As a result of the upgrade, the beamline now operates with an energy range of 7.5 x 17.5 keV, delivers 8 x 10{sup +11} photons/sec at 12.398 keV at the sample, and has an energy resolution of {delta}E/E = 1.45 x 10{sup -4} at 10 keV, which ismore » suitable for MAD experiments.« less

Authors:
; ; ; ; ; ; ; ;  [1]
  1. (UC)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1009019
Resource Type:
Conference
Resource Relation:
Conference: Ninth International Conference on Synchrotron Radiation Instrumentation;May 28 - June 2, 2006;Daegu, South Korea
Country of Publication:
United States
Language:
ENGLISH
Subject:
43 PARTICLE ACCELERATORS; 36 MATERIALS SCIENCE; ADVANCED PHOTON SOURCE; BENDING; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DESIGN; DRUGS; ENERGY RANGE; ENERGY RESOLUTION; MAGNETS; MIRRORS; PROTEINS; SYNCHROTRON RADIATION; SYNCHROTRONS

Citation Formats

Koshelev, I., Huang, R., Graber, T., Meron, M., Muir, J.L., Lavender, W., Battaile, K., Mulichak, A.M., and Keefe, L.J. Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source. United States: N. p., 2007. Web. doi:10.1063/1.2436191.
Koshelev, I., Huang, R., Graber, T., Meron, M., Muir, J.L., Lavender, W., Battaile, K., Mulichak, A.M., & Keefe, L.J. Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source. United States. doi:10.1063/1.2436191.
Koshelev, I., Huang, R., Graber, T., Meron, M., Muir, J.L., Lavender, W., Battaile, K., Mulichak, A.M., and Keefe, L.J. Tue . "Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source". United States. doi:10.1063/1.2436191.
@article{osti_1009019,
title = {Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source},
author = {Koshelev, I. and Huang, R. and Graber, T. and Meron, M. and Muir, J.L. and Lavender, W. and Battaile, K. and Mulichak, A.M. and Keefe, L.J.},
abstractNote = {Pharmaceutical research depends on macromolecular crystallography as a tool in drug design and development. To solve the de novo three-dimensional atomic structure of a protein, it is essential to know the phases of the X-rays scattered by a protein crystal. Experimental phases can be obtained from multiwavelength anomalous dispersion (MAD) experiments. Dedicated to macromolecular crystallography, the IMCA-CAT bending magnet beamline at sector 17 of the Advanced Photon Source (APS) was upgraded to provide the energy resolution required to successfully perform synchrotron radiation-based MAD phasing of protein crystal structures. A collimating mirror was inserted into the beam path upstream of a double-crystal monochromator, thus increasing the monochromatic beam throughput in a particular bandwidth without sacrificing the energy resolution of the system. The beam is focused horizontally by a sagittally bent crystal and vertically by a cylindrically bent mirror, delivering a beam at the sample of 130 {micro}m (vertically) x 250 {micro}m (horizontally) FWHM. As a result of the upgrade, the beamline now operates with an energy range of 7.5 x 17.5 keV, delivers 8 x 10{sup +11} photons/sec at 12.398 keV at the sample, and has an energy resolution of {delta}E/E = 1.45 x 10{sup -4} at 10 keV, which is suitable for MAD experiments.},
doi = {10.1063/1.2436191},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 15 00:00:00 EDT 2007},
month = {Tue May 15 00:00:00 EDT 2007}
}

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  • Pharmaceutical research depends on macromolecular crystallography as a tool in drug design and development. To solve the de novo three-dimensional atomic structure of a protein, it is essential to know the phases of the X-rays scattered by a protein crystal. Experimental phases can be obtained from multiwavelength anomalous dispersion (MAD) experiments. Dedicated to macromolecular crystallography, the IMCA-CAT bending magnet beamline at sector 17 of the Advanced Photon Source (APS) was upgraded to provide the energy resolution required to successfully perform synchrotron radiation-based MAD phasing of protein crystal structures. A collimating mirror was inserted into the beam path upstream of amore » double-crystal monochromator, thus increasing the monochromatic beam throughput in a particular bandwidth without sacrificing the energy resolution of the system. The beam is focused horizontally by a sagittally bent crystal and vertically by a cylindrically bent mirror, delivering a beam at the sample of 130 {mu}m (vertically) x 250 {mu}m (horizontally) FWHM. As a result of the upgrade, the beamline now operates with an energy range of 7.5x17.5 keV, delivers 8 x 10+11 photons/sec at 12.398 keV at the sample, and has an energy resolution of {delta}E/E = 1.45 x 10-4 at 10 keV, which is suitable for MAD experiments.« less
  • The IMCA-CAT bending-magnet beamline was upgraded with a collimating mirror in order to achieve the energy resolution required to conduct high-quality multi- and single-wavelength anomalous diffraction (MAD/SAD) experiments without sacrificing beamline flux throughput. Following the upgrade, the bending-magnet beamline achieves a flux of 8 x 10{sup 11} photons s{sup -1} at 1 {angstrom} wavelength, at a beamline aperture of 1.5 mrad (horizontal) x 86 {mu}rad (vertical), with energy resolution (limited mostly by the intrinsic resolution of the monochromator optics) {delta}E/E = 1.5 x 10{sup -4} (at 10 kV). The beamline operates in a dynamic range of 7.5-17.5 keV and deliversmore » to the sample focused beam of size (FWHM) 240 {micro}m (horizontally) x 160 {micro}m (vertically). The performance of the 17-BM beamline optics and its deviation from ideally shaped optics is evaluated in the context of the requirements imposed by the needs of protein crystallography experiments. An assessment of flux losses is given in relation to the (geometric) properties of major beamline components.« less
  • The authors describe a horizontally focusing curved-crystal monochromator that invokes a 4-point bending scheme and a liquid-metal cooling bath. The device has been designed for dispersive diffraction and spectroscopy in the 5--20 keV range, with a predicted focal spot size of {le} 100 {micro}m. To minimize thermal distortions and thermal equilibration time, the 355 x 32 x 0.8 mm crystal will be nearly half submerged in a bath of Ga-In-Sn-Zn alloy. The liquid metal thermally couples the crystal to the water-cooled Cu frame, while permitting the required crystal bending. Calculated thermal profiles and anticipated focusing properties are discussed.
  • No abstract prepared.