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Title: Structure and Function of the Ribosomal Frameshifting Pseudoknot RNA from Beet Western Yellow Virus

Abstract

Many viruses reprogram ribosomes to produce two different proteins from two different reading frames. So-called -1 frameshifting often involves pairwise alignment of two adjacent tRNAs at a 'slippery' sequence in the ribosomal A and P sites such that an overlapping codon is shifted upstream by one base relative to the zero frame. In the majority of cases, an RNA pseudoknot located downstream stimulates this type of frameshift. Crystal structures of the frameshifting RNA pseudoknot from Beet Western Yellow Virus (BWYV) have provided a detailed picture of the tertiary interactions stabilizing this folding motif, including a minor-groove triplex and quadruple-base interactions. The structure determined at atomic resolution revealed the locations of several magnesium ions and provided insights into the role of structured water stabilizing the RNA. Systematic in vitro and in vivo mutational analyses based on the structural results revealed specific tertiary interactions and regions in the pseudoknot that drastically change frameshifting efficiency. Here, we summarize recent advances in our understanding of pseudoknot-mediated ribosomal frameshifting on the basis of the insights gained from structural and functional studies of the RNA pseudoknot from BWYV.

Authors:
; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1008771
Resource Type:
Journal Article
Resource Relation:
Journal Name: Helv. Chim. Acta; Journal Volume: 86; Journal Issue: 2003
Country of Publication:
United States
Language:
ENGLISH
Subject:
36 MATERIALS SCIENCE; ALIGNMENT; BEETS; CODONS; CRYSTAL STRUCTURE; EFFICIENCY; FUNCTIONALS; IN VITRO; IN VIVO; MAGNESIUM IONS; PROTEINS; RESOLUTION; RIBOSOMES; RNA; VIRUSES; WATER

Citation Formats

Egli, M., Sarkhel, S., Minasov, G., and Rich, A. Structure and Function of the Ribosomal Frameshifting Pseudoknot RNA from Beet Western Yellow Virus. United States: N. p., 2010. Web.
Egli, M., Sarkhel, S., Minasov, G., & Rich, A. Structure and Function of the Ribosomal Frameshifting Pseudoknot RNA from Beet Western Yellow Virus. United States.
Egli, M., Sarkhel, S., Minasov, G., and Rich, A. Fri . "Structure and Function of the Ribosomal Frameshifting Pseudoknot RNA from Beet Western Yellow Virus". United States.
@article{osti_1008771,
title = {Structure and Function of the Ribosomal Frameshifting Pseudoknot RNA from Beet Western Yellow Virus},
author = {Egli, M. and Sarkhel, S. and Minasov, G. and Rich, A.},
abstractNote = {Many viruses reprogram ribosomes to produce two different proteins from two different reading frames. So-called -1 frameshifting often involves pairwise alignment of two adjacent tRNAs at a 'slippery' sequence in the ribosomal A and P sites such that an overlapping codon is shifted upstream by one base relative to the zero frame. In the majority of cases, an RNA pseudoknot located downstream stimulates this type of frameshift. Crystal structures of the frameshifting RNA pseudoknot from Beet Western Yellow Virus (BWYV) have provided a detailed picture of the tertiary interactions stabilizing this folding motif, including a minor-groove triplex and quadruple-base interactions. The structure determined at atomic resolution revealed the locations of several magnesium ions and provided insights into the role of structured water stabilizing the RNA. Systematic in vitro and in vivo mutational analyses based on the structural results revealed specific tertiary interactions and regions in the pseudoknot that drastically change frameshifting efficiency. Here, we summarize recent advances in our understanding of pseudoknot-mediated ribosomal frameshifting on the basis of the insights gained from structural and functional studies of the RNA pseudoknot from BWYV.},
doi = {},
journal = {Helv. Chim. Acta},
number = 2003,
volume = 86,
place = {United States},
year = {Fri Mar 05 00:00:00 EST 2010},
month = {Fri Mar 05 00:00:00 EST 2010}
}