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Title: Structures of Mycobacterium tuberculosis DosR and DosR-DNA Complex Involved in Gene Activation during Adaptation to Hypoxic Latency

Abstract

On encountering low oxygen conditions, DosR activates the transcription of 47 genes, promoting long-term survival of Mycobacterium tuberculosis in a non-replicating state. Here, we report the crystal structures of the DosR C-terminal domain and its complex with a consensus DNA sequence of the hypoxia-induced gene promoter. The DosR C-terminal domain contains four {alpha}-helices and forms tetramers consisting of two dimers with non-intersecting dyads. In the DNA-bound structure, each DosR C-terminal domain in a dimer places its DNA-binding helix deep into the major groove, causing two bends in the DNA. DosR makes numerous protein-DNA base contacts using only three amino acid residues per subunit: Lys179, Lys182, and Asn183. The DosR tetramer is unique among response regulators with known structures.

Authors:
; ; ; ; ;  [1]
  1. UWASH
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1008668
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Molecular Biology; Journal Volume: 354; Journal Issue: (3) ; 2005
Country of Publication:
United States
Language:
ENGLISH
Subject:
36 MATERIALS SCIENCE; AMINO ACIDS; CRYSTAL STRUCTURE; DIMERS; DNA; GENES; MYCOBACTERIUM TUBERCULOSIS; OXYGEN; RESIDUES; TRANSCRIPTION; TUBERCULOSIS

Citation Formats

Wisedchaisri, Goragot, Wu, Meiting, Rice, Adrian E, Roberts, David M, Sherman, David R, and Hol, Wim G.J.. Structures of Mycobacterium tuberculosis DosR and DosR-DNA Complex Involved in Gene Activation during Adaptation to Hypoxic Latency. United States: N. p., 2010. Web. doi:10.1016/j.jmb.2005.09.048.
Wisedchaisri, Goragot, Wu, Meiting, Rice, Adrian E, Roberts, David M, Sherman, David R, & Hol, Wim G.J.. Structures of Mycobacterium tuberculosis DosR and DosR-DNA Complex Involved in Gene Activation during Adaptation to Hypoxic Latency. United States. doi:10.1016/j.jmb.2005.09.048.
Wisedchaisri, Goragot, Wu, Meiting, Rice, Adrian E, Roberts, David M, Sherman, David R, and Hol, Wim G.J.. 2010. "Structures of Mycobacterium tuberculosis DosR and DosR-DNA Complex Involved in Gene Activation during Adaptation to Hypoxic Latency". United States. doi:10.1016/j.jmb.2005.09.048.
@article{osti_1008668,
title = {Structures of Mycobacterium tuberculosis DosR and DosR-DNA Complex Involved in Gene Activation during Adaptation to Hypoxic Latency},
author = {Wisedchaisri, Goragot and Wu, Meiting and Rice, Adrian E and Roberts, David M and Sherman, David R and Hol, Wim G.J.},
abstractNote = {On encountering low oxygen conditions, DosR activates the transcription of 47 genes, promoting long-term survival of Mycobacterium tuberculosis in a non-replicating state. Here, we report the crystal structures of the DosR C-terminal domain and its complex with a consensus DNA sequence of the hypoxia-induced gene promoter. The DosR C-terminal domain contains four {alpha}-helices and forms tetramers consisting of two dimers with non-intersecting dyads. In the DNA-bound structure, each DosR C-terminal domain in a dimer places its DNA-binding helix deep into the major groove, causing two bends in the DNA. DosR makes numerous protein-DNA base contacts using only three amino acid residues per subunit: Lys179, Lys182, and Asn183. The DosR tetramer is unique among response regulators with known structures.},
doi = {10.1016/j.jmb.2005.09.048},
journal = {Journal of Molecular Biology},
number = (3) ; 2005,
volume = 354,
place = {United States},
year = 2010,
month = 7
}
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