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Title: A bio-synthetic interface for discovery of viral entry mechanisms.

Abstract

Understanding and defending against pathogenic viruses is an important public health and biodefense challenge. The focus of our LDRD project has been to uncover the mechanisms enveloped viruses use to identify and invade host cells. We have constructed interfaces between viral particles and synthetic lipid bilayers. This approach provides a minimal setting for investigating the initial events of host-virus interaction - (i) recognition of, and (ii) entry into the host via membrane fusion. This understanding could enable rational design of therapeutics that block viral entry as well as future construction of synthetic, non-proliferating sensors that detect live virus in the environment. We have observed fusion between synthetic lipid vesicles and Vesicular Stomatitis virus particles, and we have observed interactions between Nipah virus-like particles and supported lipid bilayers and giant unilamellar vesicles.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Sandia National Laboratories
Sponsoring Org.:
USDOE
OSTI Identifier:
1008149
Report Number(s):
SAND2010-6472
TRN: US201108%%24
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; INTERFACES; LIPIDS; MEMBRANE TRANSPORT; VIRUSES

Citation Formats

Gutzler, Mike, Maar, Dianna, Negrete, Oscar, Hayden, Carl C, Sasaki, Darryl Yoshio, Stachowiak, Jeanne C, and Wang, Julia. A bio-synthetic interface for discovery of viral entry mechanisms.. United States: N. p., 2010. Web. doi:10.2172/1008149.
Gutzler, Mike, Maar, Dianna, Negrete, Oscar, Hayden, Carl C, Sasaki, Darryl Yoshio, Stachowiak, Jeanne C, & Wang, Julia. A bio-synthetic interface for discovery of viral entry mechanisms.. United States. doi:10.2172/1008149.
Gutzler, Mike, Maar, Dianna, Negrete, Oscar, Hayden, Carl C, Sasaki, Darryl Yoshio, Stachowiak, Jeanne C, and Wang, Julia. Wed . "A bio-synthetic interface for discovery of viral entry mechanisms.". United States. doi:10.2172/1008149. https://www.osti.gov/servlets/purl/1008149.
@article{osti_1008149,
title = {A bio-synthetic interface for discovery of viral entry mechanisms.},
author = {Gutzler, Mike and Maar, Dianna and Negrete, Oscar and Hayden, Carl C and Sasaki, Darryl Yoshio and Stachowiak, Jeanne C and Wang, Julia},
abstractNote = {Understanding and defending against pathogenic viruses is an important public health and biodefense challenge. The focus of our LDRD project has been to uncover the mechanisms enveloped viruses use to identify and invade host cells. We have constructed interfaces between viral particles and synthetic lipid bilayers. This approach provides a minimal setting for investigating the initial events of host-virus interaction - (i) recognition of, and (ii) entry into the host via membrane fusion. This understanding could enable rational design of therapeutics that block viral entry as well as future construction of synthetic, non-proliferating sensors that detect live virus in the environment. We have observed fusion between synthetic lipid vesicles and Vesicular Stomatitis virus particles, and we have observed interactions between Nipah virus-like particles and supported lipid bilayers and giant unilamellar vesicles.},
doi = {10.2172/1008149},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {9}
}

Technical Report:

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