skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus

Abstract

The Gram-negative aerobic eubacterium Thermus thermophilus is an extremely important thermophilic microorganism that was originally isolated from a thermal vent environment in Japan. The molybdenum cofactor in this organism is considered to be an essential component required by enzymes that catalyze diverse key reactions in the global metabolism of carbon, nitrogen and sulfur. The molybdenum-cofactor biosynthesis protein C derived from T. thermophilus was crystallized in two different space groups. Crystals obtained using the first crystallization condition belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 64.81, b = 109.84, c = 115.19 {angstrom}, {beta} = 104.9{sup o}; the crystal diffracted to a resolution of 1.9 {angstrom}. The other crystal form belonged to space group R32, with unit-cell parameters a = b = 106.57, c = 59.25 {angstrom}, and diffracted to 1.75 {angstrom} resolution. Preliminary calculations reveal that the asymmetric unit contains 12 monomers and one monomer for the crystals belonging to space group P2{sub 1} and R32, respectively.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ;  [1];  [2];  [2]
  1. (IIS)
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1007558
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallogr. F; Journal Volume: 63; Journal Issue: (1) ; 12, 2007
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; BIOSYNTHESIS; CARBON; CRYSTALLIZATION; ENZYMES; JAPAN; METABOLISM; MICROORGANISMS; MOLYBDENUM; MONOMERS; NITROGEN; PROTEINS; RESOLUTION; SPACE GROUPS; SULFUR

Citation Formats

Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Chen, Lirong, Liu, Zhi-Jie, Wang, Bi-Cheng, Nishida, Masami, Ebihara, Akio, Shinkai, Akeo, Kuramitsu, Seiki, Shiro, Yoshitsugu, Sekar, Kanagaraj, Yokoyama, Shigeyuki, SP8), and Georgia). Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus. United States: N. p., 2010. Web. doi:10.1107/S1744309106052560.
Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Chen, Lirong, Liu, Zhi-Jie, Wang, Bi-Cheng, Nishida, Masami, Ebihara, Akio, Shinkai, Akeo, Kuramitsu, Seiki, Shiro, Yoshitsugu, Sekar, Kanagaraj, Yokoyama, Shigeyuki, SP8), & Georgia). Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus. United States. doi:10.1107/S1744309106052560.
Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Chen, Lirong, Liu, Zhi-Jie, Wang, Bi-Cheng, Nishida, Masami, Ebihara, Akio, Shinkai, Akeo, Kuramitsu, Seiki, Shiro, Yoshitsugu, Sekar, Kanagaraj, Yokoyama, Shigeyuki, SP8), and Georgia). Fri . "Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus". United States. doi:10.1107/S1744309106052560.
@article{osti_1007558,
title = {Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus},
author = {Kanaujia, Shankar Prasad and Ranjani, Chellamuthu Vasuki and Jeyakanthan, Jeyaraman and Baba, Seiki and Chen, Lirong and Liu, Zhi-Jie and Wang, Bi-Cheng and Nishida, Masami and Ebihara, Akio and Shinkai, Akeo and Kuramitsu, Seiki and Shiro, Yoshitsugu and Sekar, Kanagaraj and Yokoyama, Shigeyuki and SP8) and Georgia)},
abstractNote = {The Gram-negative aerobic eubacterium Thermus thermophilus is an extremely important thermophilic microorganism that was originally isolated from a thermal vent environment in Japan. The molybdenum cofactor in this organism is considered to be an essential component required by enzymes that catalyze diverse key reactions in the global metabolism of carbon, nitrogen and sulfur. The molybdenum-cofactor biosynthesis protein C derived from T. thermophilus was crystallized in two different space groups. Crystals obtained using the first crystallization condition belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 64.81, b = 109.84, c = 115.19 {angstrom}, {beta} = 104.9{sup o}; the crystal diffracted to a resolution of 1.9 {angstrom}. The other crystal form belonged to space group R32, with unit-cell parameters a = b = 106.57, c = 59.25 {angstrom}, and diffracted to 1.75 {angstrom} resolution. Preliminary calculations reveal that the asymmetric unit contains 12 monomers and one monomer for the crystals belonging to space group P2{sub 1} and R32, respectively.},
doi = {10.1107/S1744309106052560},
journal = {Acta Crystallogr. F},
number = (1) ; 12, 2007,
volume = 63,
place = {United States},
year = {Fri Dec 03 00:00:00 EST 2010},
month = {Fri Dec 03 00:00:00 EST 2010}
}