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Title: Enclosed chambers for humidity control and sample containment in fiber diffraction

Abstract

A chamber and stretch frame for making fibers for diffraction is described. The chamber is made from a simple plastic cuvette with silicon nitride windows. It is suitable for maintaining constant humidity during fiber drying and data collection, and allows stretching of the fiber and exposure to magnetic fields during sample preparation. If necessary, it provides primary containment for toxic and infectious biological materials. The chamber has been used in fiber diffraction experiments with filamentous plant viruses and a yeast prion protein, and is shown to produce excellent orientation and to maintain hydration and order at the molecular level.

Authors:
; ; ; ;  [1];  [2]
  1. (Vanderbilt)
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1006921
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Appl. Crystallogr.; Journal Volume: 41; Journal Issue: (1) ; 02, 2008
Country of Publication:
United States
Language:
ENGLISH
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; SAMPLE HOLDERS; DESIGN; FIBERS; DIFFRACTION; HUMIDITY CONTROL; SAMPLE PREPARATION; BIOLOGICAL MATERIALS; CONTAINMENT; HYDRATION; PLANTS; SILICON NITRIDES; VIRUSES; YEASTS

Citation Formats

McDonald, M., Kendall, A., Tanaka, M., Weissman, J.S., Stubbs, G., and HHMI). Enclosed chambers for humidity control and sample containment in fiber diffraction. United States: N. p., 2008. Web. doi:10.1107/S0021889807060803.
McDonald, M., Kendall, A., Tanaka, M., Weissman, J.S., Stubbs, G., & HHMI). Enclosed chambers for humidity control and sample containment in fiber diffraction. United States. doi:10.1107/S0021889807060803.
McDonald, M., Kendall, A., Tanaka, M., Weissman, J.S., Stubbs, G., and HHMI). Mon . "Enclosed chambers for humidity control and sample containment in fiber diffraction". United States. doi:10.1107/S0021889807060803.
@article{osti_1006921,
title = {Enclosed chambers for humidity control and sample containment in fiber diffraction},
author = {McDonald, M. and Kendall, A. and Tanaka, M. and Weissman, J.S. and Stubbs, G. and HHMI)},
abstractNote = {A chamber and stretch frame for making fibers for diffraction is described. The chamber is made from a simple plastic cuvette with silicon nitride windows. It is suitable for maintaining constant humidity during fiber drying and data collection, and allows stretching of the fiber and exposure to magnetic fields during sample preparation. If necessary, it provides primary containment for toxic and infectious biological materials. The chamber has been used in fiber diffraction experiments with filamentous plant viruses and a yeast prion protein, and is shown to produce excellent orientation and to maintain hydration and order at the molecular level.},
doi = {10.1107/S0021889807060803},
journal = {J. Appl. Crystallogr.},
number = (1) ; 02, 2008,
volume = 41,
place = {United States},
year = {Mon Nov 03 00:00:00 EST 2008},
month = {Mon Nov 03 00:00:00 EST 2008}
}
  • A chamber and stretch frame for making fibers for diffraction is described. The chamber is made from a simple plastic cuvette with silicon nitride windows. It is suitable for maintaining constant humidity during fiber drying and data collection, and allows stretching of the fiber and exposure to magnetic fields during sample preparation. If necessary, it provides primary containment for toxic and infectious biological materials. The chamber has been used in fiber diffraction experiments with filamentous plant viruses and a yeast prion protein, and is shown to produce excellent orientation and to maintain hydration and order at the molecular level.
  • A new crystal-mounting method has been developed that involves a combination of controlled humid air and polymer glue for crystal coating. This method is particularly useful when applied to fragile protein crystals that are known to be sensitive to subtle changes in their physicochemical environment. Protein crystals are fragile, and it is sometimes difficult to find conditions suitable for handling and cryocooling the crystals before conducting X-ray diffraction experiments. To overcome this issue, a protein crystal-mounting method has been developed that involves a water-soluble polymer and controlled humid air that can adjust the moisture content of a mounted crystal. Bymore » coating crystals with polymer glue and exposing them to controlled humid air, the crystals were stable at room temperature and were cryocooled under optimized humidity. Moreover, the glue-coated crystals reproducibly showed gradual transformations of their lattice constants in response to a change in humidity; thus, using this method, a series of isomorphous crystals can be prepared. This technique is valuable when working on fragile protein crystals, including membrane proteins, and will also be useful for multi-crystal data collection.« less
  • Under summer conditions in areas of the USSR having hot climates, overheating is observed in space enclosed by a single-layer pneumatic structure. The effectiveness of spraying with water and painting the pneumatic structure with a compound having good reflecting characteristics has been demonstrated theoretically and experimentally. A thermal-regime calculation method is proposed for pneumatic structures employing water spraying.
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