Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex
- SVIMR-A
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a haemopoietic growth factor that acts through a ternary receptor signalling complex containing specific {alpha} (GMR{alpha}) and common {beta} ({beta}c) receptor subunits. Human GM-CSF is encoded by the gene csf2, while the genes for GMR{alpha} and {beta}c are csf2ra and csf2rb, respectively. Crystals of the ternary ectodomain complex comprising GM-CSF and the soluble extracellular regions of both the GMR{alpha} subunit and either {beta}c or its glutamine-substitution mutant N346Q were obtained using the hanging-drop vapour-diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the {beta}c subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P6{sub 3}22 and diffracted to 3.3 {angstrom} resolution.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1006714
- Journal Information:
- Acta Crystallogr. F, Vol. 64, Issue (8) ; 08, 2008; ISSN 1744-3091
- Country of Publication:
- United States
- Language:
- ENGLISH
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