Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB
Journal Article
·
· Acta Crystallogr. F
The Neisseria meningitidis outer membrane protein PorB was expressed in Escherichia coli and purified from inclusion bodies by denaturation in urea followed by refolding in buffered LDAO on a size-exclusion column. PorB has been crystallized in three different crystal forms: C222, R32 and P6{sub 3}. The C222 crystal form may contain either one or two PorB monomers in the asymmetric unit, while both the R32 and P6{sub 3} crystal forms contained one PorB monomer in the asymmetric unit. Of the three, the P6{sub 3} crystal form had the best diffraction quality, yielding data extending to 2.3 {angstrom} resolution.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1006064
- Journal Information:
- Acta Crystallogr. F, Vol. 65, Issue (10) ; 10, 2009; ISSN 1744-3091
- Country of Publication:
- United States
- Language:
- ENGLISH
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