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Title: The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria

Abstract

The contractile tail of bacteriophage T4 is a molecular machine that facilitates very high viral infection efficiency. Its major component is a tail sheath, which contracts during infection to less than half of its initial length. The sheath consists of 138 copies of the tail sheath protein, gene product (gp) 18, which surrounds the central non-contractile tail tube. The contraction of the sheath drives the tail tube through the outer membrane, creating a channel for the viral genome delivery. A crystal structure of about three quarters of gp18 has been determined and was fitted into cryo-electron microscopy reconstructions of the tail sheath before and after contraction. It was shown that during contraction, gp18 subunits slide over each other with no apparent change in their structure.

Authors:
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Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1005701
Resource Type:
Journal Article
Journal Name:
EMBO J.
Additional Journal Information:
Journal Volume: 28; Journal Issue: (7) ; 04, 2009; Journal ID: ISSN 0261-4189
Country of Publication:
United States
Language:
ENGLISH
Subject:
36 MATERIALS SCIENCE; BACTERIA; BACTERIOPHAGES; CONTRACTION; CONTRACTS; CRYSTAL STRUCTURE; DELIVERY; EFFICIENCY; GENES; LENGTH; MICROSCOPY; TUBES

Citation Formats

Aksyuk, Anastasia A., Leiman, Petr G., Kurochkina, Lidia P., Shneider, Mikhail M., Kostyuchenko, Victor A., Mesyanzhinov, Vadim V., Rossmann, Michael G., SOIBC), and Purdue). The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria. United States: N. p., 2009. Web. doi:10.1038/emboj.2009.36.
Aksyuk, Anastasia A., Leiman, Petr G., Kurochkina, Lidia P., Shneider, Mikhail M., Kostyuchenko, Victor A., Mesyanzhinov, Vadim V., Rossmann, Michael G., SOIBC), & Purdue). The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria. United States. doi:10.1038/emboj.2009.36.
Aksyuk, Anastasia A., Leiman, Petr G., Kurochkina, Lidia P., Shneider, Mikhail M., Kostyuchenko, Victor A., Mesyanzhinov, Vadim V., Rossmann, Michael G., SOIBC), and Purdue). Wed . "The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria". United States. doi:10.1038/emboj.2009.36.
@article{osti_1005701,
title = {The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria},
author = {Aksyuk, Anastasia A. and Leiman, Petr G. and Kurochkina, Lidia P. and Shneider, Mikhail M. and Kostyuchenko, Victor A. and Mesyanzhinov, Vadim V. and Rossmann, Michael G. and SOIBC) and Purdue)},
abstractNote = {The contractile tail of bacteriophage T4 is a molecular machine that facilitates very high viral infection efficiency. Its major component is a tail sheath, which contracts during infection to less than half of its initial length. The sheath consists of 138 copies of the tail sheath protein, gene product (gp) 18, which surrounds the central non-contractile tail tube. The contraction of the sheath drives the tail tube through the outer membrane, creating a channel for the viral genome delivery. A crystal structure of about three quarters of gp18 has been determined and was fitted into cryo-electron microscopy reconstructions of the tail sheath before and after contraction. It was shown that during contraction, gp18 subunits slide over each other with no apparent change in their structure.},
doi = {10.1038/emboj.2009.36},
journal = {EMBO J.},
issn = {0261-4189},
number = (7) ; 04, 2009,
volume = 28,
place = {United States},
year = {2009},
month = {7}
}