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Title: Assessment of Populus wood chemistry following the introduction of a Bt toxin gene

Abstract

Unintended changes in plant physiology, anatomy and metabolism as a result of genetic engineering are a concern as more transgenic plants are commercially deployed in the ecosystem. We compared the cell wall chemical composition of three Populus lines (Populus trichocarpa Torr. & A. Gray x Populus trichocarpa Bartr. ex Marsh., Populus trichocarpa x Populus nigra L. and Populus deltoides x Populus nigra) genetically modified to express the Cry3A or Cry3B2 protein of Bacillus thuringiensis (Bt) with the cell wall chemistry of non-transformed isogenic control lines. Three genetically modified clones, each represented by 10 independent transgenic lines, were analyzed by pyrolysis molecular beam mass spectrometry, gas chromatography/mass spectrometry and traditional wet chemical analytical methods to assess changes in cell wall composition. Based on the outcome of these techniques, there were no comprehensive differences in chemical composition between the transgenic and control lines for any of the studied clones.

Authors:
 [1];  [2];  [1];  [3];  [4]
  1. ORNL
  2. National Energy Renewable Laboratory
  3. Boise Cascade LLC
  4. Purdue University
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1003691
DOE Contract Number:
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Tree Physiology; Journal Volume: 26
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; ANATOMY; BACILLUS; CELL WALL; CHEMICAL COMPOSITION; CHEMISTRY; GENES; GENETIC ENGINEERING; MASS SPECTROSCOPY; METABOLISM; MOLECULAR BEAMS; PHYSIOLOGY; PROTEINS; PYROLYSIS; SPECTROSCOPY; TOXINS; TRANSGENIC PLANTS; WOOD

Citation Formats

Tschaplinski, Timothy J, Davis, M F, Tuskan, Gerald A, Payne, M M, and Meilan, R. Assessment of Populus wood chemistry following the introduction of a Bt toxin gene. United States: N. p., 2006. Web.
Tschaplinski, Timothy J, Davis, M F, Tuskan, Gerald A, Payne, M M, & Meilan, R. Assessment of Populus wood chemistry following the introduction of a Bt toxin gene. United States.
Tschaplinski, Timothy J, Davis, M F, Tuskan, Gerald A, Payne, M M, and Meilan, R. Sun . "Assessment of Populus wood chemistry following the introduction of a Bt toxin gene". United States. doi:.
@article{osti_1003691,
title = {Assessment of Populus wood chemistry following the introduction of a Bt toxin gene},
author = {Tschaplinski, Timothy J and Davis, M F and Tuskan, Gerald A and Payne, M M and Meilan, R},
abstractNote = {Unintended changes in plant physiology, anatomy and metabolism as a result of genetic engineering are a concern as more transgenic plants are commercially deployed in the ecosystem. We compared the cell wall chemical composition of three Populus lines (Populus trichocarpa Torr. & A. Gray x Populus trichocarpa Bartr. ex Marsh., Populus trichocarpa x Populus nigra L. and Populus deltoides x Populus nigra) genetically modified to express the Cry3A or Cry3B2 protein of Bacillus thuringiensis (Bt) with the cell wall chemistry of non-transformed isogenic control lines. Three genetically modified clones, each represented by 10 independent transgenic lines, were analyzed by pyrolysis molecular beam mass spectrometry, gas chromatography/mass spectrometry and traditional wet chemical analytical methods to assess changes in cell wall composition. Based on the outcome of these techniques, there were no comprehensive differences in chemical composition between the transgenic and control lines for any of the studied clones.},
doi = {},
journal = {Tree Physiology},
number = ,
volume = 26,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}