Assessment of Populus wood chemistry following the introduction of a Bt toxin gene

Title: Assessment of Populus wood chemistry following the introduction of a Bt toxin gene

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Abstract

Unintended changes in plant physiology, anatomy and metabolism as a result of genetic engineering are a concern as more transgenic plants are commercially deployed in the ecosystem. We compared the cell wall chemical composition of three Populus lines (Populus trichocarpa Torr. & A. Gray x Populus trichocarpa Bartr. ex Marsh., Populus trichocarpa x Populus nigra L. and Populus deltoides x Populus nigra) genetically modified to express the Cry3A or Cry3B2 protein of Bacillus thuringiensis (Bt) with the cell wall chemistry of non-transformed isogenic control lines. Three genetically modified clones, each represented by 10 independent transgenic lines, were analyzed by pyrolysis molecular beam mass spectrometry, gas chromatography/mass spectrometry and traditional wet chemical analytical methods to assess changes in cell wall composition. Based on the outcome of these techniques, there were no comprehensive differences in chemical composition between the transgenic and control lines for any of the studied clones.

Authors:

Tschaplinski, Timothy J ^[1]; Davis, M F ^[2]; Tuskan, Gerald A ^[1]; Payne, M M ^[3]; Meilan, R ^[4]

ORNL
National Energy Renewable Laboratory
Boise Cascade LLC
Purdue University

Publication Date:: Sun Jan 01 00:00:00 EST 2006

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Laboratory Directed Research and Development (LDRD) Program

OSTI Identifier:: 1003691

DOE Contract Number:: DE-AC05-00OR22725

Resource Type:: Journal Article

Resource Relation:: Journal Name: Tree Physiology; Journal Volume: 26

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; ANATOMY; BACILLUS; CELL WALL; CHEMICAL COMPOSITION; CHEMISTRY; GENES; GENETIC ENGINEERING; MASS SPECTROSCOPY; METABOLISM; MOLECULAR BEAMS; PHYSIOLOGY; PROTEINS; PYROLYSIS; SPECTROSCOPY; TOXINS; TRANSGENIC PLANTS; WOOD

Citation Formats


                    Tschaplinski, Timothy J, Davis, M F, Tuskan, Gerald A, Payne, M M, and Meilan, R. Assessment of Populus wood chemistry following the introduction of a Bt toxin gene.  United States: N. p., 2006. 
        Web.

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                    Tschaplinski, Timothy J, Davis, M F, Tuskan, Gerald A, Payne, M M, & Meilan, R. Assessment of Populus wood chemistry following the introduction of a Bt toxin gene.  United States.

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                    Tschaplinski, Timothy J, Davis, M F, Tuskan, Gerald A, Payne, M M, and Meilan, R. Sun .  
        "Assessment of Populus wood chemistry following the introduction of a Bt toxin gene".  United States.  
        doi:.

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@article{osti_1003691,

  title        = {Assessment of Populus wood chemistry following the introduction of a Bt toxin gene},

  author       = {Tschaplinski, Timothy J and Davis, M F and Tuskan, Gerald A and Payne, M M and Meilan, R},

  abstractNote = {Unintended changes in plant physiology, anatomy and metabolism as a result of genetic engineering are a concern as more transgenic plants are commercially deployed in the ecosystem. We compared the cell wall chemical composition of three Populus lines (Populus trichocarpa Torr. & A. Gray x Populus trichocarpa Bartr. ex Marsh., Populus trichocarpa x Populus nigra L. and Populus deltoides x Populus nigra) genetically modified to express the Cry3A or Cry3B2 protein of Bacillus thuringiensis (Bt) with the cell wall chemistry of non-transformed isogenic control lines. Three genetically modified clones, each represented by 10 independent transgenic lines, were analyzed by pyrolysis molecular beam mass spectrometry, gas chromatography/mass spectrometry and traditional wet chemical analytical methods to assess changes in cell wall composition. Based on the outcome of these techniques, there were no comprehensive differences in chemical composition between the transgenic and control lines for any of the studied clones.},

  doi          = {},

  journal      = {Tree Physiology},

  number       = ,

  volume       = 26,

  place        = {United States},

  year         = {Sun Jan 01 00:00:00 EST 2006},

  month        = {Sun Jan 01 00:00:00 EST 2006}

}

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Assessment of Populus Wood Chemistry Following the Introduction of a Bt Toxin Gene

Davis, M. F. ; Tuskan, G. A. ; Payne, P. ; ... - Tree Physiology

Unintended changes in plant physiology, anatomy and metabolism as a result of genetic engineering are a concern as more transgenic plants are commercially deployed in the ecosystem. We compared the cell wall chemical composition of three Populus lines (Populus trichocarpa Torr. and A. Gray x Populus deltoides Bartr. ex Marsh., Populus trichocarpa x Populus nigra L. and Populus deltoides x Populus nigra) genetically modified to express the Cry3A or Cry3B2 protein of Bacillus thuringiensis (Bt) with the cellwall chemistry of non-transformed isogenic control lines. Three genetically modified clones, each represented by 10 independent transgenic lines, were analyzed by pyrolysis molecularmore »« less
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