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Title: Automated Sampling and Imaging of Analytes Separated on Thin-Layer Chromatography Plates Using Desorption Electrospray Ionization Mass Spectrometry

Abstract

Modest modifications to the atmospheric sampling capillary of a commercial electrospray mass spectrometer and upgrades to an in-house developed surface positioning control software package (HandsFree TLC/MS ) were used to enable the automated sampling and imaging of analytes on and/or within large area surface substrates using desorption electrospray ionization mass spectrometry. Sampling and imaging of rhodamine dyes separated on TLC plates were used to illustrate some of the practical applications of this system. Examples are shown for user-defined spot sampling from separated bands on a TLC plate (one or multiple spots), scanning of a complete development lane (one or multiple lanes), or imaging of analyte bands in a development lane (i.e. multiple lane scans with close spacing). The post data processing and data display aspects of the software system are also discussed.

Authors:
 [1];  [1]
  1. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
Work for Others (WFO)
OSTI Identifier:
1003534
DOE Contract Number:
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Analytical Chemistry; Journal Volume: 78; Journal Issue: 14
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; DATA PROCESSING; DESORPTION; DYES; IONIZATION; MASS SPECTROMETERS; MASS SPECTROSCOPY; MODIFICATIONS; PLATES; POSITIONING; RHODAMINES; SAMPLING; SUBSTRATES; THIN-LAYER CHROMATOGRAPHY; Desorption electrospray ionization mass spectrometry

Citation Formats

Van Berkel, Gary J, and Kertesz, Vilmos. Automated Sampling and Imaging of Analytes Separated on Thin-Layer Chromatography Plates Using Desorption Electrospray Ionization Mass Spectrometry. United States: N. p., 2006. Web. doi:10.1021/ac060690a.
Van Berkel, Gary J, & Kertesz, Vilmos. Automated Sampling and Imaging of Analytes Separated on Thin-Layer Chromatography Plates Using Desorption Electrospray Ionization Mass Spectrometry. United States. doi:10.1021/ac060690a.
Van Berkel, Gary J, and Kertesz, Vilmos. Sun . "Automated Sampling and Imaging of Analytes Separated on Thin-Layer Chromatography Plates Using Desorption Electrospray Ionization Mass Spectrometry". United States. doi:10.1021/ac060690a.
@article{osti_1003534,
title = {Automated Sampling and Imaging of Analytes Separated on Thin-Layer Chromatography Plates Using Desorption Electrospray Ionization Mass Spectrometry},
author = {Van Berkel, Gary J and Kertesz, Vilmos},
abstractNote = {Modest modifications to the atmospheric sampling capillary of a commercial electrospray mass spectrometer and upgrades to an in-house developed surface positioning control software package (HandsFree TLC/MS ) were used to enable the automated sampling and imaging of analytes on and/or within large area surface substrates using desorption electrospray ionization mass spectrometry. Sampling and imaging of rhodamine dyes separated on TLC plates were used to illustrate some of the practical applications of this system. Examples are shown for user-defined spot sampling from separated bands on a TLC plate (one or multiple spots), scanning of a complete development lane (one or multiple lanes), or imaging of analyte bands in a development lane (i.e. multiple lane scans with close spacing). The post data processing and data display aspects of the software system are also discussed.},
doi = {10.1021/ac060690a},
journal = {Analytical Chemistry},
number = 14,
volume = 78,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}