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Title: Structural and Functional Characterization of Reston Ebola Virus VP35 Interferon Inhibitory Domain

Abstract

Ebolaviruses are causative agents of lethal hemorrhagic fever in humans and nonhuman primates. Among the filoviruses characterized thus far, Reston Ebola virus (REBOV) is the only Ebola virus that is nonpathogenic to humans despite the fact that REBOV can cause lethal disease in nonhuman primates. Previous studies also suggest that REBOV is less effective at inhibiting host innate immune responses than Zaire Ebola virus (ZEBOV) or Marburg virus. Virally encoded VP35 protein is critical for immune suppression, but an understanding of the relative contributions of VP35 proteins from REBOV and other filoviruses is currently lacking. In order to address this question, we characterized the REBOV VP35 interferon inhibitory domain (IID) using structural, biochemical, and virological studies. These studies reveal differences in double-stranded RNA binding and interferon inhibition between the two species. These observed differences are likely due to increased stability and loss of flexibility in REBOV VP35 IID, as demonstrated by thermal shift stability assays. Consistent with this finding, the 1.71-{angstrom} crystal structure of REBOV VP35 IID reveals that it is highly similar to that of ZEBOV VP35 IID, with an overall backbone r.m.s.d. of 0.64 {angstrom}, but contains an additional helical element at the linker between the two subdomainsmore » of VP35 IID. Mutations near the linker, including swapping sequences between REBOV and ZEBOV, reveal that the linker sequence has limited tolerance for variability. Together with the previously solved ligand-free and double-stranded-RNA-bound forms of ZEBOV VP35 IID structures, our current studies on REBOV VP35 IID reinforce the importance of VP35 in immune suppression. Functional differences observed between REBOV and ZEBOV VP35 proteins may contribute to observed differences in pathogenicity, but these are unlikely to be the major determinant. However, the high level of similarity in structure and the low tolerance for sequence variability, coupled with the multiple critical roles played by Ebola virus VP35 proteins, highlight the viability of VP35 as a potential target for therapeutic development.« less

Authors:
; ; ; ; ; ; ; ;  [1]
  1. Sinai
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1002715
Resource Type:
Journal Article
Journal Name:
J. Mol. Biol.
Additional Journal Information:
Journal Volume: 399; Journal Issue: (3) ; 06, 2010; Journal ID: ISSN 0022-2836
Country of Publication:
United States
Language:
ENGLISH
Subject:
36 MATERIALS SCIENCE; CRYSTAL STRUCTURE; DISEASES; FEVER; FLEXIBILITY; FUNCTIONALS; INTERFERON; MUTATIONS; PRIMATES; PROTEINS; RNA; STABILITY; TARGETS; TOLERANCE; VIABILITY

Citation Formats

Leung, Daisy W, Shabman, Reed S, Farahbakhsh, Mina, Prins, Kathleen C, Borek, Dominika M, Wang, Tianjiao, Mühlberger, Elke, Basler, Christopher F, Amarasinghe, Gaya K, BU-M), Iowa State), and UTSMC). Structural and Functional Characterization of Reston Ebola Virus VP35 Interferon Inhibitory Domain. United States: N. p., 2010. Web. doi:10.1016/j.jmb.2010.04.022.
Leung, Daisy W, Shabman, Reed S, Farahbakhsh, Mina, Prins, Kathleen C, Borek, Dominika M, Wang, Tianjiao, Mühlberger, Elke, Basler, Christopher F, Amarasinghe, Gaya K, BU-M), Iowa State), & UTSMC). Structural and Functional Characterization of Reston Ebola Virus VP35 Interferon Inhibitory Domain. United States. doi:10.1016/j.jmb.2010.04.022.
Leung, Daisy W, Shabman, Reed S, Farahbakhsh, Mina, Prins, Kathleen C, Borek, Dominika M, Wang, Tianjiao, Mühlberger, Elke, Basler, Christopher F, Amarasinghe, Gaya K, BU-M), Iowa State), and UTSMC). Tue . "Structural and Functional Characterization of Reston Ebola Virus VP35 Interferon Inhibitory Domain". United States. doi:10.1016/j.jmb.2010.04.022.
@article{osti_1002715,
title = {Structural and Functional Characterization of Reston Ebola Virus VP35 Interferon Inhibitory Domain},
author = {Leung, Daisy W and Shabman, Reed S and Farahbakhsh, Mina and Prins, Kathleen C and Borek, Dominika M and Wang, Tianjiao and Mühlberger, Elke and Basler, Christopher F and Amarasinghe, Gaya K and BU-M) and Iowa State) and UTSMC)},
abstractNote = {Ebolaviruses are causative agents of lethal hemorrhagic fever in humans and nonhuman primates. Among the filoviruses characterized thus far, Reston Ebola virus (REBOV) is the only Ebola virus that is nonpathogenic to humans despite the fact that REBOV can cause lethal disease in nonhuman primates. Previous studies also suggest that REBOV is less effective at inhibiting host innate immune responses than Zaire Ebola virus (ZEBOV) or Marburg virus. Virally encoded VP35 protein is critical for immune suppression, but an understanding of the relative contributions of VP35 proteins from REBOV and other filoviruses is currently lacking. In order to address this question, we characterized the REBOV VP35 interferon inhibitory domain (IID) using structural, biochemical, and virological studies. These studies reveal differences in double-stranded RNA binding and interferon inhibition between the two species. These observed differences are likely due to increased stability and loss of flexibility in REBOV VP35 IID, as demonstrated by thermal shift stability assays. Consistent with this finding, the 1.71-{angstrom} crystal structure of REBOV VP35 IID reveals that it is highly similar to that of ZEBOV VP35 IID, with an overall backbone r.m.s.d. of 0.64 {angstrom}, but contains an additional helical element at the linker between the two subdomains of VP35 IID. Mutations near the linker, including swapping sequences between REBOV and ZEBOV, reveal that the linker sequence has limited tolerance for variability. Together with the previously solved ligand-free and double-stranded-RNA-bound forms of ZEBOV VP35 IID structures, our current studies on REBOV VP35 IID reinforce the importance of VP35 in immune suppression. Functional differences observed between REBOV and ZEBOV VP35 proteins may contribute to observed differences in pathogenicity, but these are unlikely to be the major determinant. However, the high level of similarity in structure and the low tolerance for sequence variability, coupled with the multiple critical roles played by Ebola virus VP35 proteins, highlight the viability of VP35 as a potential target for therapeutic development.},
doi = {10.1016/j.jmb.2010.04.022},
journal = {J. Mol. Biol.},
issn = {0022-2836},
number = (3) ; 06, 2010,
volume = 399,
place = {United States},
year = {2010},
month = {9}
}