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Title: Insights into Regulated Ligand Binding Sites from the Structure of ZO-1 Src Homology 3-Guanylate Kinase Module

Abstract

Tight junctions are dynamic components of epithelial and endothelial cells that regulate the paracellular transport of ions, solutes, and immune cells. The assembly and permeability of these junctions is dependent on the zonula occludens (ZO) proteins, members of the membrane-associated guanylate kinase homolog (MAGUK) protein family, which are characterized by a core Src homology 3 (SH3)-GUK module that coordinates multiple protein-protein interactions. The structure of the ZO-1 SH3-GUK domain confirms that the interdependent folding of the SH3 and GUK domains is a conserved feature of MAGUKs, but differences in the orientation of the GUK domains in three different MAGUKs reveal interdomain flexibility of the core unit. Using pull-down assays, we show that an effector loop, the U6 region in ZO-1, forms a novel intramolecular interaction with the core module. This interaction is divalent cation-dependent and overlaps with the binding site for the regulatory molecule calmodulin on the GUK domain. These findings provide insight into the previously observed ability of the U6 region to regulate TJ assembly in vivo and the structural basis for the complex protein interactions of the MAGUK family.

Authors:
; ; ; ;  [1]
  1. UNC
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1002626
Resource Type:
Journal Article
Journal Name:
J. Biol. Chem.
Additional Journal Information:
Journal Volume: 285; Journal Issue: (18) ; 04, 2010; Journal ID: ISSN 0021-9258
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; CALMODULIN; FLEXIBILITY; IN VIVO; ORIENTATION; PERMEABILITY; PHOSPHOTRANSFERASES; PROTEINS; SOLUTES; TRANSPORT

Citation Formats

Lye, Ming F, Fanning, Alan S, Su, Ying, Anderson, James M, Lavie, Arnon, and UIC). Insights into Regulated Ligand Binding Sites from the Structure of ZO-1 Src Homology 3-Guanylate Kinase Module. United States: N. p., 2010. Web. doi:10.1074/jbc.M109.093674.
Lye, Ming F, Fanning, Alan S, Su, Ying, Anderson, James M, Lavie, Arnon, & UIC). Insights into Regulated Ligand Binding Sites from the Structure of ZO-1 Src Homology 3-Guanylate Kinase Module. United States. https://doi.org/10.1074/jbc.M109.093674
Lye, Ming F, Fanning, Alan S, Su, Ying, Anderson, James M, Lavie, Arnon, and UIC). 2010. "Insights into Regulated Ligand Binding Sites from the Structure of ZO-1 Src Homology 3-Guanylate Kinase Module". United States. https://doi.org/10.1074/jbc.M109.093674.
@article{osti_1002626,
title = {Insights into Regulated Ligand Binding Sites from the Structure of ZO-1 Src Homology 3-Guanylate Kinase Module},
author = {Lye, Ming F and Fanning, Alan S and Su, Ying and Anderson, James M and Lavie, Arnon and UIC)},
abstractNote = {Tight junctions are dynamic components of epithelial and endothelial cells that regulate the paracellular transport of ions, solutes, and immune cells. The assembly and permeability of these junctions is dependent on the zonula occludens (ZO) proteins, members of the membrane-associated guanylate kinase homolog (MAGUK) protein family, which are characterized by a core Src homology 3 (SH3)-GUK module that coordinates multiple protein-protein interactions. The structure of the ZO-1 SH3-GUK domain confirms that the interdependent folding of the SH3 and GUK domains is a conserved feature of MAGUKs, but differences in the orientation of the GUK domains in three different MAGUKs reveal interdomain flexibility of the core unit. Using pull-down assays, we show that an effector loop, the U6 region in ZO-1, forms a novel intramolecular interaction with the core module. This interaction is divalent cation-dependent and overlaps with the binding site for the regulatory molecule calmodulin on the GUK domain. These findings provide insight into the previously observed ability of the U6 region to regulate TJ assembly in vivo and the structural basis for the complex protein interactions of the MAGUK family.},
doi = {10.1074/jbc.M109.093674},
url = {https://www.osti.gov/biblio/1002626}, journal = {J. Biol. Chem.},
issn = {0021-9258},
number = (18) ; 04, 2010,
volume = 285,
place = {United States},
year = {Tue Nov 09 00:00:00 EST 2010},
month = {Tue Nov 09 00:00:00 EST 2010}
}