Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
Patent
·
OSTI ID:874322
- Pleasanton, CA
Chimeric proteins having both DNA mutation binding activity and nuclease activity are synthesized by recombinant technology. The proteins are of the general formula A-L-B and B-L-A where A is a peptide having DNA mutation binding activity, L is a linker and B is a peptide having nuclease activity. The chimeric proteins are useful for detection and identification of DNA sequence variations including DNA mutations (including DNA damage and mismatches) by binding to the DNA mutation and cutting the DNA once the DNA mutation is detected.
- Research Organization:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- DOE Contract Number:
- W-7405-ENG-48
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Number(s):
- US 6365355
- OSTI ID:
- 874322
- Country of Publication:
- United States
- Language:
- English
Mutation detection using immobilized mismatch binding protein (MutS)
|
journal | January 1995 |
[37] Affinity selection of polymerase chain reaction products by DNA-binding proteins
|
book | January 1993 |
CASTing for multicomponent DNA-binding complexes
|
journal | March 1993 |
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OSTI ID:874322