Apparatus for electrophoresis separation

Patent · Sun Jan 01 00:00:00 EST 1978

OSTI ID:863079

Anderson, Norman L ^[1]

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1. Willowbrook, IL

An apparatus is disclosed for simultaneously performing electrophoresis separations on a plurality of slab gels containing samples of protein, protein subunits or nucleic acids. A reservoir of buffer solution is divided into three compartments by two parallel partitions having vertical slots spaced along their length. A sheet of flexible, electrically insulative material is attached to each partition and is provided with vertical slits aligned with the slots. Slab-gel holders are received within the slots with the flexible material folded outwardly as flaps from the slits to overlay portions of the holder surfaces and thereby act as electrical and liquid seals. An elongated, spaghetti-like gel containing a sample of specimen that was previously separated by isoelectric focusing techniques is vertically positioned along a marginal edge portion of the slab gel. On application of an electrical potential between the two outer chambers of buffer solution, a second dimensional electrophoresis separation in accordance with molecular weight occurs as the specimen molecules migrate across the slab gel.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4088561
• OSTI ID: 863079
• Country of Publication: United States
• Language: English

Related Subjects

apparatus
electrophoresis
separation
disclosed
simultaneously
performing
separations
plurality
slab
gels
containing
samples
protein
subunits
nucleic
acids
reservoir
buffer
solution
divided
compartments
parallel
partitions
vertical
slots
spaced
length
sheet
flexible
electrically
insulative
material
attached
partition
provided
slits
aligned
slab-gel
holders
received
folded
outwardly
flaps
overlay
portions
holder
surfaces
electrical
liquid
seals
elongated
spaghetti-like
sample
specimen
previously
separated
isoelectric
focusing
techniques
vertically
positioned
marginal
edge
portion
application
potential
outer
chambers
dimensional
accordance
molecular
weight
occurs
molecules
migrate
liquid seal
electrically insulative
electrical potential
nucleic acid
molecular weight
nucleic acids
buffer solution
edge portion
slab-gel holders
electrophoresis separation
containing sample
insulative material
flexible material
vertical slit
vertical slot
electrophoresis separations
vertically position
slab-gel holder
/204/436/