Synthesis and biological incorporatin of icons into macromolecules for NMR study
Work has proceeded successfully to synthesize novel /sup 13/C-labeled materials for incorporation into macromolecules. Gram quantities of C-4 labeled uracil have been synthesized and incorporated, by means of a mutant bacterial strain into t-RNA. The t-RNA has been isolated, purified, and carbon-13 T/sub 1/ studies have begun. A modern, well equipped biochemistry laboratory has become functional during the present contract period. Good progress has been made on nonenzymatic reactions of pyridoxal-5'-phosphate with selected amino actions. This effort has successfully elucidated many reaction intermediates and products. In addition, /sup 13/C containing haptens have been synthesized and screening tests have now begun on rabbits to verify the specificity of antibodies for two haptens. (auth)
- Research Organization:
- Univ. of Utah, Salt Lake City, UT (United States)
- DOE Contract Number:
- E(11-1)-2451
- OSTI ID:
- 7355286
- Report Number(s):
- COO-2451-2; TRN: 76-013274
- Country of Publication:
- United States
- Language:
- English
Similar Records
Synthesis and biological incorporation of icons into macromolecules for NMR study. Final report, June 1, 1977--May 31, 1978
Synthesis and applications of selectively {sup 13}C-labeled RNA
Related Subjects
AMINO ACIDS
CHEMICAL REACTIONS
CARBON 13
PHOSPHORIC ACID ESTERS
POLYSACCHARIDES
LABELLING
RNA
URACILS
LABELLED COMPOUNDS
AZINES
CARBOHYDRATES
CARBON ISOTOPES
CARBOXYLIC ACIDS
ESTERS
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PYRIMIDINES
SACCHARIDES
STABLE ISOTOPES
400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials