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Title: Biodegradation of munitions compounds by a sulfate reducing bacterial enrichment culture

Abstract

The degradation of several munitions compounds was studied. The compounds included 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetraazocine, 2,4,6-trinitrobenzene (TNB), and 2,4-dinitrotoluene. All of the compounds studied were degraded by the sulfate reducing bacterial (SRB) enrichment culture. The SRB culture did not use the munitions compounds as their sole source of carbon. However, all the munitions compounds tested served as the sole source of nitrogen for the SRB culture. Degradation of munitions compounds was achieved by a co-metabolic process. The SRB culture used a variety of carbon sources including pyruvate, ethanol, formate, lactate, and H{sub 2}-CO{sub 2}. The SRB culture was an incomplete oxidizer, unable to carry out the terminal oxidation of organic substrates to CO{sub 2} as the sole product, and it did not use acetate or methanol as a carbon source. In addition to serving as nitrogen sources, the munitions compounds also served as electron acceptors in the absence of sulfate. A soil slurry experiment with 5% and 10% munitions compounds-contaminated soil showed that the contaminant TNT was metabolized by the SRB culture in the presence of pyruvate as electron donor. This culture may be useful in decontaminating munitions compounds-contaminated soil and water under anaerobic conditions.

Authors:
;  [1]
  1. Argonne National Lab., IL (United States). Environmental Research Div.
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
Department of Defense, Washington, DC (United States)
OSTI Identifier:
515534
Report Number(s):
ANL/ER/PP-86791
ON: DE97008251; TRN: AHC29718%%49
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1997]
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE; NITRO COMPOUNDS; BIODEGRADATION; SULFATE-REDUCING BACTERIA; TNT; REMEDIAL ACTION; PERFORMANCE; SOILS; EXPERIMENTAL DATA

Citation Formats

Boopathy, R, and Manning, J. Biodegradation of munitions compounds by a sulfate reducing bacterial enrichment culture. United States: N. p., 1997. Web. doi:10.2172/515534.
Boopathy, R, & Manning, J. Biodegradation of munitions compounds by a sulfate reducing bacterial enrichment culture. United States. https://doi.org/10.2172/515534
Boopathy, R, and Manning, J. 1997. "Biodegradation of munitions compounds by a sulfate reducing bacterial enrichment culture". United States. https://doi.org/10.2172/515534. https://www.osti.gov/servlets/purl/515534.
@article{osti_515534,
title = {Biodegradation of munitions compounds by a sulfate reducing bacterial enrichment culture},
author = {Boopathy, R and Manning, J},
abstractNote = {The degradation of several munitions compounds was studied. The compounds included 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetraazocine, 2,4,6-trinitrobenzene (TNB), and 2,4-dinitrotoluene. All of the compounds studied were degraded by the sulfate reducing bacterial (SRB) enrichment culture. The SRB culture did not use the munitions compounds as their sole source of carbon. However, all the munitions compounds tested served as the sole source of nitrogen for the SRB culture. Degradation of munitions compounds was achieved by a co-metabolic process. The SRB culture used a variety of carbon sources including pyruvate, ethanol, formate, lactate, and H{sub 2}-CO{sub 2}. The SRB culture was an incomplete oxidizer, unable to carry out the terminal oxidation of organic substrates to CO{sub 2} as the sole product, and it did not use acetate or methanol as a carbon source. In addition to serving as nitrogen sources, the munitions compounds also served as electron acceptors in the absence of sulfate. A soil slurry experiment with 5% and 10% munitions compounds-contaminated soil showed that the contaminant TNT was metabolized by the SRB culture in the presence of pyruvate as electron donor. This culture may be useful in decontaminating munitions compounds-contaminated soil and water under anaerobic conditions.},
doi = {10.2172/515534},
url = {https://www.osti.gov/biblio/515534}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Aug 01 00:00:00 EDT 1997},
month = {Fri Aug 01 00:00:00 EDT 1997}
}