LoTToR: An Algorithm for Missing-Wedge Correction of the Low-Tilt Tomographic 3D Reconstruction of a Single-Molecule Structure (LoTToR) v1.0
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
The low-tilt tomographic reconstruction (LoTToR) method is a post-processing method to correct the missing-wedge artifact, which contains a model-free iteration process under a set of constraints in real and reciprocal spaces. A proof of concept is conducted by using the LoTToR on a phantom, i.e., a simulated 3D reconstruction from a low-tilt series of images, including that within a tilt range of ±15°. The method is validated by using both negative-staining (NS) and cryo-electron tomography (cryo-ET) experimental data. A significantly reduced missing-wedge artifact verifies the capability of LoTToR, suggesting a new tool to support the future study of macromolecular dynamics, fluctuation and chemical activity from the viewpoint of single-molecule 3D structure determination. Moreover, the software can be used for hospital computer tomography (CT) to reduce the radiation damage to patients without reducing the CT image quality.
- Short Name / Acronym:
- LoTToR: An Algorithm for Missing-Wedge Correction of the Low-Tilt Tomographic 3D Reconstruction of a Single-Molecule Structure
- Project Type:
- Open Source, No Publicly Available Repository
- Site Accession Number:
- 2021-048
- Software Type:
- Scientific
- License(s):
- BSD 3-clause "New" or "Revised" License
- Research Organization:
- Xi'an University of Science and Technology; Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE; NIH - National Institutes of Health; National Natural Science Foundation of China (NSFC)Primary Award/Contract Number:AC02-05CH11231Other Award/Contract Number:R01 HL115153 (AWD2743), R01 GM104427 (AWD5)
11504290
- DOE Contract Number:
- AC02-05CH11231
- Code ID:
- 67216
- OSTI ID:
- 1832012
- Country of Origin:
- United States
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