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Title: Expression of eukaryotic polypeptides in chloroplasts

Abstract

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

Inventors:
Publication Date:
Research Org.:
Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176724
Patent Number(s):
RE44266
Application Number:
11/197,730; 6,156,517
Assignee:
The Scripps Research Institutes (La Jolla, CA)
DOE Contract Number:  
FG03-93ER20116
Resource Type:
Patent
Resource Relation:
Patent File Date: 2005 Aug 03
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mayfield, Stephen P. Expression of eukaryotic polypeptides in chloroplasts. United States: N. p., 2013. Web.
Mayfield, Stephen P. Expression of eukaryotic polypeptides in chloroplasts. United States.
Mayfield, Stephen P. 2013. "Expression of eukaryotic polypeptides in chloroplasts". United States. https://www.osti.gov/servlets/purl/1176724.
@article{osti_1176724,
title = {Expression of eukaryotic polypeptides in chloroplasts},
author = {Mayfield, Stephen P.},
abstractNote = {The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.},
doi = {},
url = {https://www.osti.gov/biblio/1176724}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 04 00:00:00 EDT 2013},
month = {Tue Jun 04 00:00:00 EDT 2013}
}

Works referenced in this record:

Single polarization transmission in pedestal-supported silicon waveguides
journal, January 2011