[Molecular, genetic and physiological analysis of photoinhibition and photosynthetic]. Progress report, June 1991--November 1992

Technical Report · Tue Dec 01 00:00:00 EST 1992

DOI:https://doi.org/10.2172/10191478· OSTI ID:10191478

A major goal of this project is to use a combined molecular genetic, biochemical and physiological approach to understand the relationship between photosynthetic performance and the structure of the multifunctional D1 reaction center protein of Photosystem II encoded by the chloroplast psbA gene. Relative to other chloroplast proteins, turover of D1 is rapid and highly light dependent and de novo synthesis of D1 is required for a plant`s recovery from short term exposure to irradiances which induce photoinhibitory damage. These observations have led to models for a damage/repair cycle of PSII involving the targeted degradation and replacement of photodamaged D1. To investigate the effects of perturbing the D1 cycle on photosynthesis and autotrophic growth under high and low irradiance, we have examined the consequences of site-specific mutations of the psbA and 16S rRNA genes affecting synthesis, maturation and function/stability of the D1 protein introduced into the chloroplast genome of wildtype strain of the green alga Chlamydomonas reinhardtii using biolistic transformation.

• Research Organization: Duke Univ., Durham, NC (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG05-89ER14005
• OSTI ID: 10191478
• Report Number(s): DOE/ER/14005-T2; ON: DE93004365
• Resource Relation: Other Information: PBD: [1992]
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
14 SOLAR ENERGY
THYLAKOID MEMBRANE PROTEINS
GENE MUTATIONS
PHOSPHORYLATION
PROGRESS REPORT
PHOTOSYNTHETIC REACTION CENTERS
RNA PROCESSING
DNA SEQUENCING
RIBOSOMAL RNA
CARBON 13
TRACER TECHNIQUES
BIOLOGICAL HALF-LIFE
SULFUR 35
MUTANTS
OXYGEN 18
550201
140505
PHOTOCHEMICAL, PHOTOBIOLOGICAL, AND THERMOCHEMICAL CONVERSION