skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Abstract

Compositions and methods of producing components of protein biosynthetic machinery that include glutamyl orthogonal tRNAs, glutamyl orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of glutamyl tRNAs/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.

Inventors:
 [1];  [2];  [3]
  1. San Francisco, CA
  2. La Jolla, CA
  3. Cambridge, MA
Publication Date:
Research Org.:
The Scripps Research Institute (US)
Sponsoring Org.:
USDOE
OSTI Identifier:
988159
Patent Number(s):
7,527,943
Application Number:
10/563,656
Assignee:
The Scripps Research Institute (La Jolla, CA)
DOE Contract Number:  
FG03-00ER45812
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Anderson, J Christopher, Schultz, Peter G, and Santoro, Stephen. Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof. United States: N. p., 2009. Web.
Anderson, J Christopher, Schultz, Peter G, & Santoro, Stephen. Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof. United States.
Anderson, J Christopher, Schultz, Peter G, and Santoro, Stephen. 2009. "Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof". United States. https://www.osti.gov/servlets/purl/988159.
@article{osti_988159,
title = {Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof},
author = {Anderson, J Christopher and Schultz, Peter G and Santoro, Stephen},
abstractNote = {Compositions and methods of producing components of protein biosynthetic machinery that include glutamyl orthogonal tRNAs, glutamyl orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of glutamyl tRNAs/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.},
doi = {},
url = {https://www.osti.gov/biblio/988159}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 05 00:00:00 EDT 2009},
month = {Tue May 05 00:00:00 EDT 2009}
}

Works referenced in this record:

Enzymatic Phosphorylation of Unnatural Nucleosides
journal, December 2002


Quadruplet codons: implications for code expansion and the specification of translation step size
journal, April 2000


Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999


Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria
journal, January 2001


Four-base codons ACCA, ACCU and ACCC are recognized by frameshift suppressor sufJ
journal, August 1981


Exploring the Limits of Codon and Anticodon Size
journal, February 2002


Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion
journal, May 2003


An unnatural base pair for incorporating amino acid analogs into proteins
journal, February 2002


Domain-specific recruitment of amide amino acids for protein synthesis
journal, September 2000


Actions of the anticodon arm in translation on the phenotypes of RNA mutants
journal, November 1986


An expanded genetic code with a functional quadruplet codon
journal, May 2004


In vitro protein engineering using synthetic tRNAAla with different anticodons
journal, August 1993


An Expanded Eukaryotic Genetic Code
journal, August 2003


Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002

  • Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
  • Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027
  • https://doi.org/10.1021/ja027007w

Adaptation of an Orthogonal Archaeal Leucyl-tRNA and Synthetase Pair for Four-base, Amber, and Opal Suppression
journal, August 2003


Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002


Efficient Incorporation of Nonnatural Amino Acids with Large Aromatic Groups into Streptavidin in In Vitro Protein Synthesizing Systems
journal, January 1999


A general approach for the generation of orthogonal tRNAs
journal, September 2001


Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli
journal, March 2001


An efficient system for the evolution of aminoacyl-tRNA synthetase specificity
journal, September 2002


Incorporation of non-natural amino acids into proteins
journal, December 2002


Incorporation of Two Different Nonnatural Amino Acids Independently into a Single Protein through Extension of the Genetic Code
journal, December 1999


A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins
journal, May 2000


How glutaminyl-tRNA synthetase selects glutamine
journal, April 1998


Architectures of class-defining and specific domains of glutamyl-tRNA synthetase
journal, March 1995


Five-base codons for incorporation of nonnatural amino acids into proteins
journal, September 2001


Characterization of an ‘orthogonal’ suppressor tRNA derived from E. coli tRNA2Gln
journal, September 1997


Programming peptidomimetic syntheses by translating genetic codes designed de novo
journal, May 2003


A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase.
journal, April 1990


Expanding tRNA recognition of a tRNA synthetase by a single amino acid change
journal, May 2003


Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002


Expanding the Genetic Code of Escherichia coli
journal, April 2001


Reading frame selection and transfer RNA anticodon loop stacking
journal, December 1987


Novel transfer RNAs that are active in Escherichia coli
journal, May 1992


Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells
journal, November 2002