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Title: Methods and materials for the production of L-lactic acid in yeast

Patent ·
OSTI ID:988145
 [1];  [2];  [3]
  1. Jordan, MN
  2. Minnetonka, MN
  3. Maple Grove, MN
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Recombinant yeast are provided having, in one aspect, multiple exogenous LDH genes integrated into the genome, while leaving native PDC genes intact. In a second aspect, recombinant yeast are provided having an exogenous LDH gene integrated into its genome at the locus of a native PDC gene, with deletion of the native PDC gene. The recombinant yeast are useful in fermentation process for producing lactic acid.

Research Organization:
Cargill, Inc. (Wayzata, MN)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC36-00GO10598
Assignee:
Cargill, Inc. (Wayzata, MN)
Patent Number(s):
7,534,597
Application Number:
10/452,038
OSTI ID:
988145
Country of Publication:
United States
Language:
English

References (11)

Efficient Homolactic Fermentation by Kluyveromyces lactis Strains Defective in Pyruvate Utilization and Transformed with the Heterologous LDH Gene journal December 2001
Identification of a gene encoding the pyruvate decarboxylase gene regulator CaPdc2p fromCandida albicans journal May 1999
Cloning, characterization and insertional inactivation of the Lactobacillus helveticus D(?) lactate dehydrogenase gene journal June 1994
Development of metabolically engineered Saccharomyces cerevisiae cells for the production of lactic acid journal May 1995
Structure and Function of L-Lactate Dehydrogenases from Thermophilic and Mesophilic Bacteria VII. Nucleotide Sequence of the Lactate Dehydrogenase Gene from the Mesophilic Bacterium Bacillus megaterium. Preparation and Properties of a Hybrid Lactate Dehydrogenase Comprising Moieties of the B. megaterium and B. stearothermophilus Enzymes journal January 1987
A deletion of the PDC1 gene for pyruvate decarboxylase of yeast causes a different phenotype than previously isolated point mutations journal February 1989
Genetic manipulation of the pathogenic yeast Candida parapsilosis journal October 2002
Characterisation of PDC2, a gene necessary for high level expression of pyruvate decarboxylase structural genes in Saccharomyces cerevlsiae journal December 1993
Metalloprotease Inhibitors GM6001 and TAPI-0 Inhibit the Obligate Intracellular Human Pathogen Chlamydia trachomatis by Targeting Peptide Deformylase of the Bacterium journal March 2006
Identification, cloning and characterisation of a new gene required for full pyruvate decarboxylase activity in Saccharomyces cerevisiae journal March 1989
The isolation and nucleotide sequence of the pyruvate decarboxylase gene from Kluyveromyces marxianus journal September 1993

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