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Title: Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same

Patent ·
OSTI ID:984485
 [1];  [2]
  1. La Jolla, CA
  2. San Diego, CA
+ Show Author Affiliations

Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors.

Research Organization:
Univ. of California (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-01ER63276
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
7,393,923
Application Number:
10/984,694
OSTI ID:
984485
Country of Publication:
United States
Language:
English

References (23)

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Human Activation-Induced Cytidine Deaminase Is Induced by IL-4 and Negatively Regulated by CD45: Implication of CD45 as a Janus Kinase Phosphatase in Antibody Diversification journal February 2003
Genome-wide somatic hypermutation journal April 2004
Fluorescent proteins from nonbioluminescent Anthozoa species journal October 1999
Somatic Hypermutation in the Heavy Chain Locus Correlates with Transcription journal July 1998
A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins journal December 2001
AID mutates E. coli suggesting a DNA deamination mechanism for antibody diversification journal July 2002
Hypermutation targets a green fluorescent protein-encoding transgene in the presence of immunoglobulin enhancers journal April 1999
Somatic hypermutation of the AID transgene in B and non-B cells journal August 2002
GFP-like Proteins as Ubiquitous Metazoan Superfamily: Evolution of Functional Features and Structural Complexity journal May 2004
Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas journal January 2002
Somatic hypermutation of immunoglobulin and non–immunoglobulin genes
  • Storb, Ursula; Ming Shen, Hong; Michael, Nancy
  • Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 356, Issue 1405 https://doi.org/10.1098/rstb.2000.0743
journal January 2001
RNA Editing Enzyme APOBEC1 and Some of Its Homologs Can Act as DNA Mutators journal November 2002
The generation of antibody diversity through somatic hypermutation and class switch recombination journal January 2004
The roots of antibody diversity journal September 2002
Crystal Structure of the Aequorea victoria Green Fluorescent Protein journal September 1996
Death by Deamination: A Novel Host Restriction System for HIV-1 journal August 2003
TdT-Accessible Breaks Are Scattered over the Immunoglobulin V Domain in a Constitutively Hypermutating B Cell Line journal December 1998
Ablation of XRCC2/3 transforms immunoglobulin V gene conversion into somatic hypermutation journal August 2001
Increased Transcription Levels Induce Higher Mutation Rates in a Hypermutating Cell Line journal April 2001
Evolutionary and somatic selection of the antibody repertoire in the mouse journal November 1987
The TATA binding protein, c-Myc and survivin genes are not somatically hypermutated, while Ig and BCL6 genes are hypermutated in human memory B cells journal July 2000
A monomeric red fluorescent protein journal June 2002

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