Method of inactivation of an end product of energy metabolism in Zymomonas mobilis

Zhang, Min; Chou, Yat-Chen

Title: Method of inactivation of an end product of energy metabolism in Zymomonas mobilis

Patent · Tue May 20 00:00:00 EDT 2008

OSTI ID:984338

Zhang, Min ^[1]; Chou, Yat-Chen ^[1]

Lakewood, CO

The present invention briefly provides a method of site-specific insertion in Zymomonas, comprising, providing a Zymomonas gene fragment, interrupting a DNA sequence the fragment, and transforming the Zymomonas through homologous recombination with the interrupted fragment.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Midwest Research Institute, Kansas City, MO (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC36-99GO10337

Assignee:: Midwest Research Institute (Kansas City, MO)

Patent Number(s):: 7,374,939

Application Number:: 09/562,613

OSTI ID:: 984338

Country of Publication:: United States

Language:: English

References (10)

Transposon mutagenesis and strain construction in *Zymomonas mobilis* Pappas, K. ‐M.; Galani, I.; Typas, M. A. Journal of Applied Microbiology, Vol. 82, Issue 3 https://doi.org/10.1046/j.1365-2672.1997.00376.x	journal	March 1997
Structural Analysis of Tn5 Auerswald, E. -A.; Ludwig, G.; Schaller, H. Cold Spring Harbor Symposia on Quantitative Biology, Vol. 45, Issue 0 https://doi.org/10.1101/SQB.1981.045.01.019	journal	January 1981
The IdhA Gene Encoding the Fermentative Lactate Dehydrogenase of Escherichia Coli Bunch, P. K.; Mat-Jan, F.; Lee, N. Microbiology, Vol. 143, Issue 1 https://doi.org/10.1099/00221287-143-1-187	journal	January 1997
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis Zhang, M.; Eddy, C.; Deanda, K. Science, Vol. 267, Issue 5195 https://doi.org/10.1126/science.267.5195.240	journal	January 1995
Factors affecting transposition activity of IS50 and Tn5 ends Dodson, Karen W.; Berg, Douglas E. Gene, Vol. 76, Issue 2 https://doi.org/10.1016/0378-1119(89)90161-3	journal	March 1989
Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria Fellay, Rémy; Frey, Joachim; Krisch, Henry Gene, Vol. 52, Issue 2-3 https://doi.org/10.1016/0378-1119(87)90041-2	journal	January 1987
Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering. Deanda, K.; Zhang, M.; Eddy, C. Applied and environmental microbiology, Vol. 62, Issue 12 https://doi.org/10.1128/AEM.62.12.4465-4470.1996	journal	January 1996
Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. Herrero, M.; de Lorenzo, V.; Timmis, K. N. Journal of Bacteriology, Vol. 172, Issue 11 https://doi.org/10.1128/JB.172.11.6557-6567.1990	journal	November 1990
Conditions that promote production of lactic acid byZymomonas mobilis in batch and continuous culture Lawford, Hugh G.; Rousseau, Joyce D. Applied Biochemistry and Biotechnology, Vol. 70-72, Issue 1 https://doi.org/10.1007/BF02920134	journal	March 1998
Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli. Yomano, L. P.; Scopes, R. K.; Ingram, L. O. Journal of Bacteriology, Vol. 175, Issue 13 https://doi.org/10.1128/JB.175.13.3926-3933.1993	journal	January 1993

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