Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster
We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95percent of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using Phi C31 integrase to rescue mutations. They can be modified through recombineering, for example to incorporate protein tags and assess expression patterns.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- Life Sciences Division
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 962653
- Report Number(s):
- LBNL-1979E; TRN: US200916%%301
- Journal Information:
- Nature Methods, Vol. 6, Issue 6; Related Information: Journal Publication Date: 2008
- Country of Publication:
- United States
- Language:
- English
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