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Title: Genome, transcriptome, and secretome analysis of wood decay fungus postia placenta supports unique mechanisms of lignocellulose conversion

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America
OSTI ID:960750
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  1. Los Alamos National Laboratory
  2. CLARK UNIV
  3. CLARK UNIV.
  4. UNIV WIEN
  5. CIB, CSIC, MADRID
  6. FOREST PRODUCTS LAB
  7. U. WISCONSIN
  8. DOE JGI
  9. U. CATHOLICA DE CHILE
  10. U. CINCINATTI
  11. PUBLIC U. NAVARRE
  12. U. TORONTO
  13. CNRS, MARSEILLE
  14. NOVOZYMES, INC.
  15. PNNL
  16. MASCOMA, INC.
  17. GEORG-AUGUST-U.
  18. GEORG-AUGUST-U
  19. UNM
  20. MCMASTER U.
  21. CHARLES U.
  22. NOVOZYMES
+ Show Author Affiliations

Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative {beta}-1-4 endoglucanase were expressed at high levels relative to glucose grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC{center_dot}MSIMS). Also upregulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H202. These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H202 react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons to the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC52-06NA25396
OSTI ID:
960750
Report Number(s):
LA-UR-08-07047; LA-UR-08-7047; PNASA6; TRN: US201008%%673
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America; ISSN 0027-8424
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BENZOQUINONES
CARBON SOURCES
CELLULOSE
CHEMISTRY
CONVERSION
DECAY
DEPOLYMERIZATION
DIVERSIFICATION
ECOSYSTEMS
ENZYMES
FORESTS
FUNGI
GENES
GENETICS
GLUCOSE
GLYCOSIDES
GROWTH
HYDROLASES
HYDROXYL RADICALS
IRON
LEVELS
LIGNIN
LIQUIDS
MASS SPECTROSCOPY
OXIDASES
OXIDIZERS
OXIDOREDUCTASES
PEPTIDES
PHANEROCHAETE
WOOD