Raman Spectroscopy of DNA Packaging in Individual Human Sperm Cells distinguishes Normal from Abnormal Cells

Huser, T; Orme, C; Hollars, C; Corzett, M; Balhorn, R

doi:10.1002/jbio.200910012

Title: Raman Spectroscopy of DNA Packaging in Individual Human Sperm Cells distinguishes Normal from Abnormal Cells

Journal Article · Mon Mar 09 00:00:00 EDT 2009 · Journal of Biophotonics, vol. 2, no. 5, May 1, 2009, pp. 322-332

DOI:https://doi.org/10.1002/jbio.200910012· OSTI ID:957626

Huser, T; Orme, C; Hollars, C; Corzett, M; Balhorn, R

Healthy human males produce sperm cells of which about 25-40% have abnormal head shapes. Increases in the percentage of sperm exhibiting aberrant sperm head morphologies have been correlated with male infertility, and biochemical studies of pooled sperm have suggested that sperm with abnormal shape may contain DNA that has not been properly repackaged by protamine during spermatid development. We have used micro-Raman spectroscopy to obtain Raman spectra from individual human sperm cells and examined how differences in the Raman spectra of sperm chromatin correlate with cell shape. We show that Raman spectra of individual sperm cells contain vibrational marker modes that can be used to assess the efficiency of DNA-packaging for each cell. Raman spectra obtained from sperm cells with normal shape provide evidence that DNA in these sperm is very efficiently packaged. We find, however, that the relative protein content per cell and DNA packaging efficiencies are distributed over a relatively wide range for sperm cells with both normal and abnormal shape. These findings indicate that single cell Raman spectroscopy should be a valuable tool in assessing the quality of sperm cells for in-vitro fertilization.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-48

OSTI ID:: 957626

Report Number(s):: LLNL-JRNL-411195; TRN: US201016%%68

Journal Information:: Journal of Biophotonics, vol. 2, no. 5, May 1, 2009, pp. 322-332, Vol. 2, Issue 5

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
CHROMATIN
DNA
EFFICIENCY
FERTILIZATION
IN VITRO
MALES
PACKAGING
PROTAMINES
PROTEINS
RAMAN SPECTRA
RAMAN SPECTROSCOPY
SHAPE
SPECTROSCOPY
SPERMATOZOA

Title: Raman Spectroscopy of DNA Packaging in Individual Human Sperm Cells distinguishes Normal from Abnormal Cells

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