Sequence finishing and mapping of Drosophila melanogasterheterochromatin
Genome sequences for most metazoans are incomplete due tothe presence of repeated DNA in the pericentromeric heterochromatin. Theheterochromatic regions of D. melanogaster contain 20 Mb of sequenceamenable to mapping, sequence assembly and finishing. Here we describethe generation of 15 Mb of finished or improved heterochromatic sequenceusing available clone resources and assembly and mapping methods. We alsoconstructed a BAC-based physical map that spans approximately 13 Mb ofthe pericentromeric heterochromatin, and a cytogenetic map that positionsapproximately 11 Mb of BAC contigs and sequence scaffolds in specificchromosomal locations. The integrated sequence assembly and maps greatlyimprove our understanding of the structure and composition of this poorlyunderstood fraction of a metazoan genome and provide a framework forfunctional analyses.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Director, Office of Science; National Institutes ofHealth
- DOE Contract Number:
- DE-AC02-05CH11231; NIH5 R01HG000747-13
- OSTI ID:
- 928724
- Report Number(s):
- LBNL-62840; SCEHDK; R&D Project: L0446; BnR: 400412000; TRN: US200811%%308
- Journal Information:
- Science, Vol. 316, Issue 5831; Related Information: Journal Publication Date: June 15,2007; ISSN 0193-4511
- Country of Publication:
- United States
- Language:
- English
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