MICROFLUIDIC MIXERS FOR THE INVESTIGATION OF PROTEIN FOLDING USING SYNCHROTRON RADIATION CIRCULAR DICHROISM SPECTROSCOPY

Kane, A; Hertzog, D; Baumgartel, P; Lengefeld, J; Horsley, D; Schuler, B; Bakajin, O

doi:10.1021/ac801764r

Title: MICROFLUIDIC MIXERS FOR THE INVESTIGATION OF PROTEIN FOLDING USING SYNCHROTRON RADIATION CIRCULAR DICHROISM SPECTROSCOPY

Conference · Mon Mar 20 00:00:00 EST 2006

DOI:https://doi.org/10.1021/ac801764r· OSTI ID:928522

Kane, A; Hertzog, D; Baumgartel, P; Lengefeld, J; Horsley, D; Schuler, B; Bakajin, O

The purpose of this study is to design, fabricate and optimize microfluidic mixers to investigate the kinetics of protein secondary structure formation with Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy. The mixers are designed to rapidly initiate protein folding reaction through the dilution of denaturant. The devices are fabricated out of fused silica, so that they are transparent in the UV. We present characterization of mixing in the fabricated devices, as well as the initial SRCD data on proteins inside the mixers.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-48

OSTI ID:: 928522

Report Number(s):: UCRL-PROC-220084; TRN: US0803280

Resource Relation:: Journal Volume: 80; Journal Issue: 24; Conference: Presented at: Hilton Head 2006 Workshop, Hilton Head, SC, United States, Jun 04 - Jun 08, 2006

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
47 OTHER INSTRUMENTATION
DESIGN
DICHROISM
DILUTION
KINETICS
MIXERS
PROTEINS
SILICA
SPECTROSCOPY
SYNCHROTRON RADIATION

Title: MICROFLUIDIC MIXERS FOR THE INVESTIGATION OF PROTEIN FOLDING USING SYNCHROTRON RADIATION CIRCULAR DICHROISM SPECTROSCOPY

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