RNA editing in Drosophila melanogaster: new targets and functionalconsequences
Abstract
Adenosine deaminases that act on RNA (ADARs) catalyze the site-specific conversion of adenosine to inosine in primary mRNA transcripts. These re-coding events affect coding potential, splice-sites, and stability of mature mRNAs. ADAR is an essential gene and studies in mouse, C. elegans, and Drosophila suggest its primary function is to modify adult behavior by altering signaling components in the nervous system. By comparing the sequence of isogenic cDNAs to genomic DNA, we have identified and experimentally verified 27 new targets of Drosophila ADAR. Our analyses lead us to identify new classes of genes whose transcripts are targets of ADAR including components of the actin cytoskeleton, and genes involved in ion homeostasis and signal transduction. Our results indicate that editing in Drosophila increases the diversity of the proteome, and does so in a manner that has direct functional consequences on protein function.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Director, Office of Science; National Institutes ofHealth
- OSTI Identifier:
- 919373
- Report Number(s):
- LBNL-59973
R&D Project: L0604; BnR: YN0100000; TRN: US200822%%258
- DOE Contract Number:
- DE-AC02-05CH11231; NIHHG002673
- Resource Type:
- Journal Article
- Journal Name:
- RNA Society
- Additional Journal Information:
- Journal Volume: 12; Related Information: Journal Publication Date: 2006
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ACTIN; ADENOSINE; ADULTS; DNA; DROSOPHILA; FUNCTIONALS; GENES; HOMEOSTASIS; INOSINE; NERVOUS SYSTEM; PROTEINS; STABILITY; TARGETS
Citation Formats
Stapleton, Mark, Carlson, Joseph W, and Celniker, Susan E. RNA editing in Drosophila melanogaster: new targets and functionalconsequences. United States: N. p., 2006.
Web. doi:10.1261/rna.254306.
Stapleton, Mark, Carlson, Joseph W, & Celniker, Susan E. RNA editing in Drosophila melanogaster: new targets and functionalconsequences. United States. https://doi.org/10.1261/rna.254306
Stapleton, Mark, Carlson, Joseph W, and Celniker, Susan E. 2006.
"RNA editing in Drosophila melanogaster: new targets and functionalconsequences". United States. https://doi.org/10.1261/rna.254306. https://www.osti.gov/servlets/purl/919373.
@article{osti_919373,
title = {RNA editing in Drosophila melanogaster: new targets and functionalconsequences},
author = {Stapleton, Mark and Carlson, Joseph W and Celniker, Susan E},
abstractNote = {Adenosine deaminases that act on RNA (ADARs) catalyze the site-specific conversion of adenosine to inosine in primary mRNA transcripts. These re-coding events affect coding potential, splice-sites, and stability of mature mRNAs. ADAR is an essential gene and studies in mouse, C. elegans, and Drosophila suggest its primary function is to modify adult behavior by altering signaling components in the nervous system. By comparing the sequence of isogenic cDNAs to genomic DNA, we have identified and experimentally verified 27 new targets of Drosophila ADAR. Our analyses lead us to identify new classes of genes whose transcripts are targets of ADAR including components of the actin cytoskeleton, and genes involved in ion homeostasis and signal transduction. Our results indicate that editing in Drosophila increases the diversity of the proteome, and does so in a manner that has direct functional consequences on protein function.},
doi = {10.1261/rna.254306},
url = {https://www.osti.gov/biblio/919373},
journal = {RNA Society},
number = ,
volume = 12,
place = {United States},
year = {Tue Sep 05 00:00:00 EDT 2006},
month = {Tue Sep 05 00:00:00 EDT 2006}
}