Real-time Bacterial Detection by Single Cell Based Sensors UsingSynchrotron FTIR Spectromicroscopy
Microarrays of single macrophage cell based sensors weredeveloped and demonstrated for real time bacterium detection bysynchrotron FTIR microscopy. The cells were patterned on gold-SiO2substrates via a surface engineering technique by which the goldelectrodes were immobilized with fibronectin to mediate cell adhesion andthe silicon oxide background were passivated with PEG to resist proteinadsorption and cell adhesion. Cellular morphology and IR spectra ofsingle, double, and triple cells on gold electrodes exposed tolipopolysaccharide (LPS) of different concentrations were compared toreveal the detection capabilities of these biosensors. The single-cellbased sensors were found to generate the most significant IR wave numbervariation and thus provide the highest detection sensitivity. Changes inmorphology and IR spectrum for single cells exposed to LPS were found tobe time- and concentration-dependent and correlated with each other verywell. FTIR spectra from single cell arrays of gold electrodes withsurface area of 25 mu-m2, 100 mu-m2, and 400 mu-m2 were acquired usingboth synchrotron and conventional FTIR spectromicroscopes to study thesensitivity of detection. The results indicated that the developedsingle-cell platform can be used with conventional FTIRspectromicroscopy. This technique provides real-time, label-free, andrapid bacterial detection, and may allow for statistic and highthroughput analyses, and portability.
- Research Organization:
- COLLABORATION - U.Washington
- Sponsoring Organization:
- USDOE Director, Office of Science. Office of Basic EnergySciences; National Science Foundation (NSF)
- DOE Contract Number:
- DE-AC02-05CH11231; NSF:EEC-9872882 ANDEEC-9529161
- OSTI ID:
- 918655
- Report Number(s):
- LBNL-58699; R&D Project: 458121; BnR: KC0204016; TRN: US0805873
- Journal Information:
- Biosensors&Bioelectronics, Vol. 23, Issue 2; Related Information: Journal Publication Date: September2007
- Country of Publication:
- United States
- Language:
- English
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