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Title: Development of porous polymer monoliths for reverse-phase chromatography of proteins.

Abstract

The polymers developed in this project are intended for use as a stationary phase in reverse-phase chromatography of proteins, where the mobile phase is a solution of acetonitrile and a phosphate buffer, 6.6 pH. A full library of pore sizes have been developed ranging from 0.41{micro}m to 4.09 {micro}m; these pore sizes can be determined by the solvent ratio of tetrahydrofuran:methoxyethanol during polymerization. A column that can separate proteins in an isocratic mode would be a vast improvement from the common method of separating proteins through gradient chromatography using multiple solvents. In the stationary phase, the main monomers have hydrophobic tails, lauryl acrylate and steryl acrylate. Separations of small hydrophobic molecules and peptides (trial molecules) have efficiencies of 24,000-33,000 theoretical plates m{sup -1}. The combination of a highly non-polar stationary phase and a mobile phase where the polarity can be controlled provide for excellent separation.

Authors:
 [1];  [1]
  1. Sandia National Laboratories, Albuquerque, NM
Publication Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
918341
Report Number(s):
SAND2003-8517
TRN: US200818%%353
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ACETONITRILE; ACRYLATES; CHROMATOGRAPHY; MONOMERS; PEPTIDES; PHOSPHATES; PLATES; POLYMERIZATION; POLYMERS; PROTEINS; SOLVENTS

Citation Formats

Shepodd, Timothy J, and Stephens, Christopher P. Development of porous polymer monoliths for reverse-phase chromatography of proteins.. United States: N. p., 2003. Web. doi:10.2172/918341.
Shepodd, Timothy J, & Stephens, Christopher P. Development of porous polymer monoliths for reverse-phase chromatography of proteins.. United States. https://doi.org/10.2172/918341
Shepodd, Timothy J, and Stephens, Christopher P. 2003. "Development of porous polymer monoliths for reverse-phase chromatography of proteins.". United States. https://doi.org/10.2172/918341. https://www.osti.gov/servlets/purl/918341.
@article{osti_918341,
title = {Development of porous polymer monoliths for reverse-phase chromatography of proteins.},
author = {Shepodd, Timothy J and Stephens, Christopher P},
abstractNote = {The polymers developed in this project are intended for use as a stationary phase in reverse-phase chromatography of proteins, where the mobile phase is a solution of acetonitrile and a phosphate buffer, 6.6 pH. A full library of pore sizes have been developed ranging from 0.41{micro}m to 4.09 {micro}m; these pore sizes can be determined by the solvent ratio of tetrahydrofuran:methoxyethanol during polymerization. A column that can separate proteins in an isocratic mode would be a vast improvement from the common method of separating proteins through gradient chromatography using multiple solvents. In the stationary phase, the main monomers have hydrophobic tails, lauryl acrylate and steryl acrylate. Separations of small hydrophobic molecules and peptides (trial molecules) have efficiencies of 24,000-33,000 theoretical plates m{sup -1}. The combination of a highly non-polar stationary phase and a mobile phase where the polarity can be controlled provide for excellent separation.},
doi = {10.2172/918341},
url = {https://www.osti.gov/biblio/918341}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Sep 01 00:00:00 EDT 2003},
month = {Mon Sep 01 00:00:00 EDT 2003}
}