Genetically modified CHO cells for studying the genotoxicity of heterocyclic amines from cooked foods
Conference
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OSTI ID:90679
We have developed metabolically competent CHO cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with IQ but not with PhIP. This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States); National Cancer Inst., Bethesda, MD (United States)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 90679
- Report Number(s):
- UCRL-JC-121703; CONF-950741-1; ON: DE95016065; CNN: Grant CA55861; TRN: 95:017895
- Resource Relation:
- Conference: 7. congress of the International Union of Toxicology and 7th international congress of toxicology - horizons in toxicology: preparing for the 21st century, Seattle, WA (United States), 2-7 Jul 1995; Other Information: PBD: Jul 1995
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:90679