Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change
Patent
·
OSTI ID:903351
- Danville, CA
- Severna Park, MD
- Oakland, CA
Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.
- Research Organization:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-48
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Number(s):
- 7,157,232
- Application Number:
- 11/020,731
- OSTI ID:
- 903351
- Country of Publication:
- United States
- Language:
- English
Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.
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journal | August 1991 |
DNA Electrochemical Behaviors, Recognition and Sensing by Combining with PCR Technique
|
journal | June 2003 |
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