High Throughput Technologies for Functional Analysis of Archael Genomics
The specific aims of this project were as follows: (1) to design primers to each predicted open reading frame (ORF) in M. jannaschii and M. thermoautotrophicum to allow the amplification of a unique target sequence that will represent the corresponding coding region on a complete genome chip (2) to amplify each target sequence from M. jannaschii and M. thermoautotrophicum and verify that these PCR products are the expected DNA fragment (3) to establish a relational database that will track the production of target DNAs and the nucleotide sequence used to represent each ORF.
- Research Organization:
- The Institute for Genomic Research
- Sponsoring Organization:
- USDOE - Office of Energy Research (ER)
- DOE Contract Number:
- FG02-98ER20319
- OSTI ID:
- 899965
- Report Number(s):
- DOE/ER/20319-1; TRN: US200714%%172
- Country of Publication:
- United States
- Language:
- English
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