Promoting uranium immobilization by the activities of microbial phophatases
The first objective of this project is to determine the relationship of phosphatase activity to metal resistance in subsurface strains and the role of lateral gene transfer (LGT) in dissemination of nonspecific acid phosphatase genes. Nonspecific acid phosphohydrolases are a broad group of secreted microbial phosphatases that function in acidic-to-neutral pH ranges and utilize a wide range of organophosphate substrates. We have previously shown that PO43- accumulation during growth on a model organophosphorus compound was attributable to the overproduction of alkaline phosphatase by genetically modified subsurface pseudomonads [Powers et al. (2002) FEMS Microbiol. Ecol. 41:115-123]. During this report period, we have extended these results to include indigenous metal resistant subsurface microorganisms cultivated from the Field Research Center (FRC), in Oak Ridge Tennessee.
- Research Organization:
- Georgia Institute of Technology, Atlanta, GA
- Sponsoring Organization:
- USDOE Office of Science (SC)
- OSTI ID:
- 893417
- Report Number(s):
- NABIR-1024775-2005; R&D Project: NABIR 1024775; TRN: US200625%%269
- Country of Publication:
- United States
- Language:
- English
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Promoting uranium immobilization by the activities of microbial phophatases
Promoting Uranium Immobilization by the Activities of Microbial Phosphatases