Genome-wide analysis of Polycomb targets in Drosophila
Polycomb Group (PcG) complexes are multiprotein assemblages that bind to chromatin and establish chromatin states leading to epigenetic silencing. PcG proteins regulate homeotic genes in flies and vertebrates but little is known about other PcG targets and the role of the PcG in development, differentiation and disease. We have determined the distribution of the PcG proteins PC, E(Z) and PSC and of histone H3K27 trimethylation in the Drosophila genome. At more than 200 PcG target genes, binding sites for the three PcG proteins colocalize to presumptive Polycomb Response Elements (PREs). In contrast, H3 me3K27 forms broad domains including the entire transcription unit and regulatory regions. PcG targets are highly enriched in genes encoding transcription factors but receptors, signaling proteins, morphogens and regulators representing all major developmental pathways are also included.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Director. Office of Science. Office of Biological andEnvironmental Research; National Institutes of Health. National HumanGenome Research Institute, National Institute of General Medical SciencesGrant GM704403
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 891634
- Report Number(s):
- LBNL-60004; R&D Project: GTLADM; BnR: KP1102010; TRN: US200622%%276
- Journal Information:
- Nature Genetics, Vol. 38; Related Information: Journal Publication Date: 2006
- Country of Publication:
- United States
- Language:
- English
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