Plasma deposited diamond-like carbon films for large neutralarrays
Abstract
To understand how large systems of neurons communicate, we need to develop methods for growing patterned networks of large numbers of neurons. We have found that diamond-like carbon thin films formed by energetic deposition from a filtered vacuum arc carbon plasma can serve as ''neuron friendly'' substrates for the growth of large neural arrays. Lithographic masks can be used to form patterns of diamond-like carbon, and regions of selective neuronal attachment can form patterned neural arrays. In the work described here, we used glass microscope slides as substrates on which diamond-like carbon was deposited. PC-12 rat neurons were then cultured on the treated substrates and cell growth monitored. Neuron growth showed excellent contrast, with prolific growth on the treated surfaces and very low growth on the untreated surfaces. Here we describe the vacuum arc plasma deposition technique employed, and summarize results demonstrating that the approach can be used to form large patterns of neurons.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE; Royal Golden Jubliee. Thailand ResearchFund
- OSTI Identifier:
- 886979
- Report Number(s):
- LBNL-56394
R&D Project: Z2N197; BnR: NN4101010; TRN: US200617%%418
- DOE Contract Number:
- DE-AC02-05CH11231
- Resource Type:
- Conference
- Resource Relation:
- Conference: 10th International Conference and School onPlasma Physics and Controlled Fusion, Alushta (Crimea), Ukraine,09/13-18/2004
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; CARBON; DEPOSITION; EDUCATIONAL FACILITIES; GLASS; MICROSCOPES; NERVE CELLS; PHYSICS; PLASMA; SUBSTRATES; THIN FILMS
Citation Formats
Brown, I G, Blakely, E A, Bjornstad, K A, Galvin, J E, Monteiro, O R, and Sangyuenyongpipat, S. Plasma deposited diamond-like carbon films for large neutralarrays. United States: N. p., 2004.
Web.
Brown, I G, Blakely, E A, Bjornstad, K A, Galvin, J E, Monteiro, O R, & Sangyuenyongpipat, S. Plasma deposited diamond-like carbon films for large neutralarrays. United States.
Brown, I G, Blakely, E A, Bjornstad, K A, Galvin, J E, Monteiro, O R, and Sangyuenyongpipat, S. 2004.
"Plasma deposited diamond-like carbon films for large neutralarrays". United States. https://www.osti.gov/servlets/purl/886979.
@article{osti_886979,
title = {Plasma deposited diamond-like carbon films for large neutralarrays},
author = {Brown, I G and Blakely, E A and Bjornstad, K A and Galvin, J E and Monteiro, O R and Sangyuenyongpipat, S},
abstractNote = {To understand how large systems of neurons communicate, we need to develop methods for growing patterned networks of large numbers of neurons. We have found that diamond-like carbon thin films formed by energetic deposition from a filtered vacuum arc carbon plasma can serve as ''neuron friendly'' substrates for the growth of large neural arrays. Lithographic masks can be used to form patterns of diamond-like carbon, and regions of selective neuronal attachment can form patterned neural arrays. In the work described here, we used glass microscope slides as substrates on which diamond-like carbon was deposited. PC-12 rat neurons were then cultured on the treated substrates and cell growth monitored. Neuron growth showed excellent contrast, with prolific growth on the treated surfaces and very low growth on the untreated surfaces. Here we describe the vacuum arc plasma deposition technique employed, and summarize results demonstrating that the approach can be used to form large patterns of neurons.},
doi = {},
url = {https://www.osti.gov/biblio/886979},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jul 15 00:00:00 EDT 2004},
month = {Thu Jul 15 00:00:00 EDT 2004}
}