Automated parallel capillary electrophoretic system
Abstract
An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.
- Inventors:
- Publication Date:
- Research Org.:
- Spectrumedix Corporation, State College, PA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 879540
- Patent Number(s):
- 6,027,627
- Application Number:
- 09/105,988
- Assignee:
- Spectrumedix Corporation (State College, PA)
- DOE Contract Number:
- FG02-97ER82461
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 1998 Jun 29
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Li, Qingbo, Kane, Thomas E., Liu, Changsheng, Sonnenschein, Bernard, Sharer, Michael V., and Kernan, John R. Automated parallel capillary electrophoretic system. United States: N. p., 2000.
Web.
Li, Qingbo, Kane, Thomas E., Liu, Changsheng, Sonnenschein, Bernard, Sharer, Michael V., & Kernan, John R. Automated parallel capillary electrophoretic system. United States.
Li, Qingbo, Kane, Thomas E., Liu, Changsheng, Sonnenschein, Bernard, Sharer, Michael V., and Kernan, John R. 2000.
"Automated parallel capillary electrophoretic system". United States. https://www.osti.gov/servlets/purl/879540.
@article{osti_879540,
title = {Automated parallel capillary electrophoretic system},
author = {Li, Qingbo and Kane, Thomas E. and Liu, Changsheng and Sonnenschein, Bernard and Sharer, Michael V. and Kernan, John R.},
abstractNote = {An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.},
doi = {},
url = {https://www.osti.gov/biblio/879540},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 22 00:00:00 EST 2000},
month = {Tue Feb 22 00:00:00 EST 2000}
}