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Title: New self-assembled nanocrystal micelles for biolabels and biosensors.

Abstract

The ability of semiconductor nanocrystals (NCs) to display multiple (size-specific) colors simultaneously during a single, long term excitation holds great promise for their use in fluorescent bio-imaging. The main challenges of using nanocrystals as biolabels are achieving biocompatibility, low non-specific adsorption, and no aggregation. In addition, functional groups that can be used to further couple and conjugate with biospecies (proteins, DNAs, antibodies, etc.) are required. In this project, we invented a new route to the synthesis of water-soluble and biocompatible NCs. Our approach is to encapsulate as-synthesized, monosized, hydrophobic NCs within the hydrophobic cores of micelles composed of a mixture of surfactants and phospholipids containing head groups functionalized with polyethylene glycol (-PEG), -COOH, and NH{sub 2} groups. PEG provided biocompatibility and the other groups were used for further biofunctionalization. The resulting water-soluble metal and semiconductor NC-micelles preserve the optical properties of the original hydrophobic NCs. Semiconductor NCs emit the same color; they exhibit equal photoluminescence (PL) intensity under long-time laser irradiation (one week) ; and they exhibit the same PL lifetime (30-ns). The results from transmission electron microscopy and confocal fluorescent imaging indicate that water-soluble semiconductor NC-micelles are biocompatible and exhibit no aggregation in cells. We have extended the surfactant/lipidmore » encapsulation techniques to synthesize water-soluble magnetic NC-micelles. Transmission electron microscopy results suggest that water-soluble magnetic NC-micelles exhibit no aggregation. The resulting NC-micelles preserve the magnetic properties of the original hydrophobic magnetic NCs. Viability studies conducted using yeast cells suggest that the magnetic nanocrystal-micelles are biocompatible. We have demonstrated, for the first time, that using external oscillating magnetic fields to manipulate the magnetic micelles, we can kill live cells, presenting a new magnetodynamic therapy without side effects.« less

Authors:
;  [1];  [1]; ; ;  [2];  [2]
  1. University of New Mexico, Albuquerque, NM
  2. University of New Mexico, Albuquerque, NM
Publication Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
877147
Report Number(s):
SAND2005-7134
TRN: US200606%%780
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADSORPTION; ANTIBODIES; ENCAPSULATION; IRRADIATION; MAGNETIC FIELDS; MAGNETIC PROPERTIES; OPTICAL PROPERTIES; PHOSPHOLIPIDS; PHOTOLUMINESCENCE; POLYETHYLENE GLYCOLS; SIDE EFFECTS; SYNTHESIS; TRANSMISSION ELECTRON MICROSCOPY; YEASTS; Nanocrystals.; Self-assembly.; Biosensors.

Citation Formats

Tallant, David Robert, Wilson, Michael C., Leve, Erik W., Fan, Hongyou, Brinker, C. Jeffrey, Gabaldon, John, and Scullin, Chessa. New self-assembled nanocrystal micelles for biolabels and biosensors.. United States: N. p., 2005. Web. doi:10.2172/877147.
Tallant, David Robert, Wilson, Michael C., Leve, Erik W., Fan, Hongyou, Brinker, C. Jeffrey, Gabaldon, John, & Scullin, Chessa. New self-assembled nanocrystal micelles for biolabels and biosensors.. United States. https://doi.org/10.2172/877147
Tallant, David Robert, Wilson, Michael C., Leve, Erik W., Fan, Hongyou, Brinker, C. Jeffrey, Gabaldon, John, and Scullin, Chessa. 2005. "New self-assembled nanocrystal micelles for biolabels and biosensors.". United States. https://doi.org/10.2172/877147. https://www.osti.gov/servlets/purl/877147.
@article{osti_877147,
title = {New self-assembled nanocrystal micelles for biolabels and biosensors.},
author = {Tallant, David Robert and Wilson, Michael C. and Leve, Erik W. and Fan, Hongyou and Brinker, C. Jeffrey and Gabaldon, John and Scullin, Chessa},
abstractNote = {The ability of semiconductor nanocrystals (NCs) to display multiple (size-specific) colors simultaneously during a single, long term excitation holds great promise for their use in fluorescent bio-imaging. The main challenges of using nanocrystals as biolabels are achieving biocompatibility, low non-specific adsorption, and no aggregation. In addition, functional groups that can be used to further couple and conjugate with biospecies (proteins, DNAs, antibodies, etc.) are required. In this project, we invented a new route to the synthesis of water-soluble and biocompatible NCs. Our approach is to encapsulate as-synthesized, monosized, hydrophobic NCs within the hydrophobic cores of micelles composed of a mixture of surfactants and phospholipids containing head groups functionalized with polyethylene glycol (-PEG), -COOH, and NH{sub 2} groups. PEG provided biocompatibility and the other groups were used for further biofunctionalization. The resulting water-soluble metal and semiconductor NC-micelles preserve the optical properties of the original hydrophobic NCs. Semiconductor NCs emit the same color; they exhibit equal photoluminescence (PL) intensity under long-time laser irradiation (one week) ; and they exhibit the same PL lifetime (30-ns). The results from transmission electron microscopy and confocal fluorescent imaging indicate that water-soluble semiconductor NC-micelles are biocompatible and exhibit no aggregation in cells. We have extended the surfactant/lipid encapsulation techniques to synthesize water-soluble magnetic NC-micelles. Transmission electron microscopy results suggest that water-soluble magnetic NC-micelles exhibit no aggregation. The resulting NC-micelles preserve the magnetic properties of the original hydrophobic magnetic NCs. Viability studies conducted using yeast cells suggest that the magnetic nanocrystal-micelles are biocompatible. We have demonstrated, for the first time, that using external oscillating magnetic fields to manipulate the magnetic micelles, we can kill live cells, presenting a new magnetodynamic therapy without side effects.},
doi = {10.2172/877147},
url = {https://www.osti.gov/biblio/877147}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Dec 01 00:00:00 EST 2005},
month = {Thu Dec 01 00:00:00 EST 2005}
}