Microfabricated electrochemiluminescence cell for chemical reaction detection

Patent · Wed Jan 01 00:00:00 EST 2003

OSTI ID:875055

Northrup, M Allen ^[1]; Hsueh, Yun-Tai ^[2]; Smith, Rosemary L ^[2]

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1. Berkeley, CA
2. Davis, CA

A detector cell for a silicon-based or non-silicon-based sleeve type chemical reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The detector cell is an electrochemiluminescence cell constructed of layers of silicon with a cover layer of glass, with spaced electrodes located intermediate various layers forming the cell. The cell includes a cavity formed therein and fluid inlets for directing reaction fluid therein. The reaction chamber and detector cell may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The ECL cell may also be used in synthesis instruments, particularly those for DNA amplification and synthesis.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• Assignee: The Regents of the University of Calfornia (Oakland, CA)
• Patent Number(s): US 6521181
• OSTI ID: 875055
• Country of Publication: United States
• Language: English

Related Subjects

microfabricated
electrochemiluminescence
cell
chemical
reaction
detection
detector
silicon-based
non-silicon-based
sleeve
type
chamber
combines
heaters
doped
polysilicon
heating
bulk
silicon
convection
cooling
constructed
layers
cover
layer
glass
spaced
electrodes
located
intermediate
forming
cavity
formed
fluid
inlets
directing
utilized
synthesis
processing
organic
inorganic
biochemical
reactions
polymerase
chain
pcr
andor
dna
ligase
examples
synthetic
thermal-cycling-based
ecl
instruments
amplification
reaction chamber
chemical reaction
bulk silicon
detector cell
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