Method for altering antibody light chain interactions
Abstract
A method for recombinant antibody subunit dimerization including modifying at least one codon of a nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in the interface segment of the light polypeptide variable region, the charged amino acid having a first polarity; and modifying at least one codon of the nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in an interface segment of the heavy polypeptide variable region corresponding to a position in the light polypeptide variable region, the charged amino acid having a second polarity opposite the first polarity. Nucleic acid sequences which code for novel light chain proteins, the latter of which are used in conjunction with the inventive method, are also provided.
- Inventors:
-
- Naperville, IL
- Evanston, IL
- Elmhurst, IL
- Downers Grove, IL
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 874898
- Patent Number(s):
- US 6485943
- Assignee:
- The University of Chicago (Chicago, IL)
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; altering; antibody; light; chain; interactions; recombinant; subunit; dimerization; including; modifying; codon; nucleic; acid; sequence; replace; amino; occurring; naturally; charged; position; interface; segment; polypeptide; variable; region; polarity; heavy; opposite; sequences; code; novel; proteins; latter; conjunction; inventive; provided; nucleic acid; amino acid; acid sequence; inventive method; /435/530/
Citation Formats
Stevens, Fred J, Stevens, Priscilla Wilkins, Raffen, Rosemarie, and Schiffer, Marianne. Method for altering antibody light chain interactions. United States: N. p., 2002.
Web.
Stevens, Fred J, Stevens, Priscilla Wilkins, Raffen, Rosemarie, & Schiffer, Marianne. Method for altering antibody light chain interactions. United States.
Stevens, Fred J, Stevens, Priscilla Wilkins, Raffen, Rosemarie, and Schiffer, Marianne. 2002.
"Method for altering antibody light chain interactions". United States. https://www.osti.gov/servlets/purl/874898.
@article{osti_874898,
title = {Method for altering antibody light chain interactions},
author = {Stevens, Fred J and Stevens, Priscilla Wilkins and Raffen, Rosemarie and Schiffer, Marianne},
abstractNote = {A method for recombinant antibody subunit dimerization including modifying at least one codon of a nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in the interface segment of the light polypeptide variable region, the charged amino acid having a first polarity; and modifying at least one codon of the nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in an interface segment of the heavy polypeptide variable region corresponding to a position in the light polypeptide variable region, the charged amino acid having a second polarity opposite the first polarity. Nucleic acid sequences which code for novel light chain proteins, the latter of which are used in conjunction with the inventive method, are also provided.},
doi = {},
url = {https://www.osti.gov/biblio/874898},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
Works referenced in this record:
Novel arrangement of immunoglobulin variable domains: x-ray crystallographic analysis of the .lambda.-chain dimer Bence-Jones protein Loc
journal, August 1985
- Chang, Chong Hwan; Short, Michael T.; Westholm, Florence A.
- Biochemistry, Vol. 24, Issue 18
Structural and Dynamic Properties of the Fv Fragment and the Single-Chain Fv Fragment of an Antibody in Solution Investigated by Heteronuclear 3D NMR Spectroscopy
journal, March 1994
- Freund, Christian; Ross, Alfred; Plueckthun, Andreas
- Biochemistry, Vol. 33, Issue 11
Self-association of human immunoglobulin kappa I light chains: role of the third hypervariable region.
journal, February 1980
- Stevens, F. J.; Westholm, F. A.; Solomon, A.
- Proceedings of the National Academy of Sciences, Vol. 77, Issue 2
Refined crystal structure of a recombinant immunoglobulin domain and a complementarity-determining region 1-grafted mutant
journal, June 1992
- Steipe, Boris; Plückthun, Andreas; Huber, Robert
- Journal of Molecular Biology, Vol. 225, Issue 3
Bacterial expression of immunoglobulin fragments
journal, January 1993
- Skerra, Arne
- Current Opinion in Immunology, Vol. 5, Issue 2
Computer simulation of protein self-association during small-zone gel filtration. Estimation of equilibrium constants
journal, April 1981
- Stevens, F. J.; Schiffer, M.
- Biochemical Journal, Vol. 195, Issue 1
Mutational effects on inclusion body formation in the periplasmic expression of the immunoglobulin VL domain REI
journal, April 1996
- Chan, Winnie; Helms, Larry R.; Brooks, Ian
- Folding and Design, Vol. 1, Issue 2
Antibody engineering: the use of Escherichia coli as an expression host
journal, April 1992
- Ward, E. Sally
- The FASEB Journal, Vol. 6, Issue 7
Reengineering immunoglobulin domain interactions by introduction of charged residues
journal, April 1998
- Raffen, R.; Stevens, P. W.; Boogaard, C.
- Protein Engineering Design and Selection, Vol. 11, Issue 4
Dual conformations of an immunoglobulin light-chain dimer: heterogeneity of antigen specificity and idiotope profile may result from multiple variable-domain interaction mechanisms.
journal, September 1988
- Stevens, F. J.; Chang, C. H.; Schiffer, M.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 18
Analysis of protein-protein interaction by simulation of small-zone size exclusion chromatography. Stochastic formulation of kinetic rate contributions to observed high-performance liquid chromatography elution characteristics
journal, June 1989
- Stevens, F. J.
- Biophysical Journal, Vol. 55, Issue 6
Antibody Engineering: Advances From the Use of Escherichia coli Expression Systems
journal, June 1991
- Plückthun, Andreas
- Nature Biotechnology, Vol. 9, Issue 6
Renaturation, Purification and Characterization of Recombinant Fab-Fragments Produced in Escherichia coli
journal, February 1991
- Buchner, Johannes; Rudolph, Rainer
- Nature Biotechnology, Vol. 9, Issue 2
Structure of a second crystal form of Bence-Jones protein Loc: strikingly different domain associations in two crystal forms of a single protein
journal, May 1989
- Schiffer, M.; Ainsworth, C.; Xu, Z. B.
- Biochemistry, Vol. 28, Issue 9