Expression of multiple proteins in transgenic plants

Patent · Tue Jan 01 00:00:00 EST 2002

OSTI ID:874759

Vierstra, Richard D ^[1]; Walker, Joseph M ^[1]

---

1. Madison, WI

A method is disclosed for the production of multiple proteins in transgenic plants. A DNA construct for introduction into plants includes a provision to express a fusion protein of two proteins of interest joined by a linking domain including plant ubiquitin. When the fusion protein is produced in the cells of a transgenic plant transformed with the DNA construction, native enzymes present in plant cells cleave the fusion protein to release both proteins of interest into the cells of the transgenic plant. Since the proteins are produced from the same fusion protein, the initial quantities of the proteins in the cells of the plant are approximately equal.

• Research Organization: The Consortium for Plant Biotechnology Research, Inc., West Lafayette, IN (US)
• DOE Contract Number: FC05-92OR22072
• Assignee: Wisconsin Alumni Research Foundation (Madison, WI)
• Patent Number(s): US 6455759
• OSTI ID: 874759
• Country of Publication: United States
• Language: English

Related Subjects

expression
multiple
proteins
transgenic
plants
method
disclosed
production
dna
construct
introduction
provision
express
fusion
protein
joined
linking
domain
including
plant
ubiquitin
produced
cells
transformed
construction
native
enzymes
cleave
release
initial
quantities
approximately
equal
plant cell
dna construct
fusion protein
transgenic plant
/800/