Nucleic acid amplification using modular branched primers

Ulanovsky, Levy; Raja, Mugasimangalam C

Title: Nucleic acid amplification using modular branched primers

Patent · Mon Jan 01 00:00:00 EST 2001

OSTI ID:873589

Ulanovsky, Levy ^[1]; Raja, Mugasimangalam C ^[2]

Westmont, IL
Downers Grove, IL

Methods and compositions expand the options for making primers for use in amplifying nucleic acid segments. The invention eliminates the step of custom synthesis of primers for Polymerase Chain Reactions (PCR). Instead of being custom-synthesized, a primer is replaced by a combination of several oligonucleotide modules selected from a pre-synthesized library. A modular combination of just a few oligonucleotides essentially mimics the performance of a conventional, custom-made primer by matching the sequence of the priming site in the template. Each oligonucleotide module has a segment that matches one of the stretches within the priming site.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

DOE Contract Number:: W-31109-ENG-38

Assignee:: University of Chicago (Chicago, IL)

Patent Number(s):: US 6197556

OSTI ID:: 873589

Country of Publication:: United States

Language:: English

References (18)

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Title: Nucleic acid amplification using modular branched primers

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References (18)

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